HPLC Calibration was done in Reverse Phase, but not in
normal phase why explain?
- 11 Answers
- 30045 Views
- Cipla, Orchid, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / prabhakaran.g.s
Reverse phase and Normal phase classification is based on
the mobile phase and stationary phase (column).But we're
calibrating the HPLC for PUMP,COLUMN OVEN,AUTOINJECTOR and
DETECTOR etc.So we can perform the calibration for HPLC
with Normal phase also.But the ease availability of column
and mobile phase of Reversed phase, it is widely use for
the HPLC calibration.
| Is This Answer Correct ? | 96 Yes | 7 No |
Answer / d.sambireddy
Reverse phase and Normal phase related to the mobile phase
and column.
But we are calibrating the HPLC Instument all parameters in
Reverse phase calibration.it is equally suitable to Normal
phase analysis
No need to check to normal phase calibration
| Is This Answer Correct ? | 28 Yes | 2 No |
Answer / achyut
calibration -related with the total performance of hplc system
pump,flow , injection , accuracy & so on
in case of reverse phase, system suitability performed with well accuracy & precision because in rp volatile organic
phases are not used so the chances of getting accuacy is more
than np
| Is This Answer Correct ? | 6 Yes | 4 No |
Answer / pramod prajapati
Reverse phase and Normal Phase is related to mobile phase
and stationary phase used in HPLC,but here we are
calibrating HPLC and can be done in any phase.
| Is This Answer Correct ? | 3 Yes | 2 No |
Answer / deepak pal
WE CALIBRATE HPLC FOR REVERSE PHASE BCOZ ONLY MOBILE PHASE
IS DIFFERENT IN NORMAL PHASE AND WE USE MOSTLY REVERSE
PHASE CHROMATOGRAPHIC TECHNIQUES FOR ANALYSING SAMPLES AND
OTHER CONDITIONS REMAIN ALMOST SAME FOR NORMAL PHASE
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / b srinu
HPLC Calibration is a parameter of instrument performance in normal phase we changed column and mobile phase.
normal phase calibration not required. if we monitoring normal phase we can perform normal phase calibration.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / sandeepkatakam
due to cost effective we are doing in RP.otherwise we can
do in both
| Is This Answer Correct ? | 4 Yes | 6 No |
Answer / zahir
In reverse phase it is easy to calibrate hplc than normal phase bec in normal phase n haxane is used it v highly evapurate so it also change in the area of the sample as time passes and n hexane is break septa of vial so we preferred reverse phase than normal phase.
| Is This Answer Correct ? | 0 Yes | 4 No |
Answer / oumspn
coz caffeine is nonpolar & we have to take rp hplc
| Is This Answer Correct ? | 0 Yes | 8 No |
In my analysis by HPLC,In sample main peak and placebo peak are eluting with shorter resolution ( almost look like doublet). how can i get max resolution ? Is changing gradient method is usefull?
The boiling point of 1,3 butadiene is -4.4 DC & the boiling point of Pentane is 36.1 DC. Then how come Pentane elute before 1,3 BD in GC analysis?
why we use actone for Gpv test in hplc calibration
What is the difference between Spectroscopy and spectrophotometry,What are the applications?
4 Answers Caplin Point, Cipla,
how v can identify functional groups by wet techniques?
What is (Q) point dissolution?
What is the difference between bulk property detectors and Solute property detectors vivimed labs limited
What is the solvent/material used to balance spirit level in analytical balance?
5 Answers Glenmark, MMC Health Care,
what is difference between IR & FTIR ? how we calibarte both ? I want to know the procedure.
How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?
what is mean by extactable and leachable study?
what is the difference between loss on drying in ordinary oven technique and vacuum oven technique
4 Answers Divis Laboratories, Ranbaxy,
Organic Chemistry 
