Interested to Buy Any Domain ? << Click Here >> for more details...
HPLC Calibration was done in Reverse Phase, but not in
normal phase why explain?
- 11 Answers
- 33977 Views
- Cipla, Orchid, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / prabhakaran.g.s
Reverse phase and Normal phase classification is based on
the mobile phase and stationary phase (column).But we're
calibrating the HPLC for PUMP,COLUMN OVEN,AUTOINJECTOR and
DETECTOR etc.So we can perform the calibration for HPLC
with Normal phase also.But the ease availability of column
and mobile phase of Reversed phase, it is widely use for
the HPLC calibration.
| Is This Answer Correct ? | 96 Yes | 7 No |
Answer / d.sambireddy
Reverse phase and Normal phase related to the mobile phase
and column.
But we are calibrating the HPLC Instument all parameters in
Reverse phase calibration.it is equally suitable to Normal
phase analysis
No need to check to normal phase calibration
| Is This Answer Correct ? | 28 Yes | 2 No |
Answer / achyut
calibration -related with the total performance of hplc system
pump,flow , injection , accuracy & so on
in case of reverse phase, system suitability performed with well accuracy & precision because in rp volatile organic
phases are not used so the chances of getting accuacy is more
than np
| Is This Answer Correct ? | 6 Yes | 4 No |
Answer / pramod prajapati
Reverse phase and Normal Phase is related to mobile phase
and stationary phase used in HPLC,but here we are
calibrating HPLC and can be done in any phase.
| Is This Answer Correct ? | 3 Yes | 2 No |
Answer / deepak pal
WE CALIBRATE HPLC FOR REVERSE PHASE BCOZ ONLY MOBILE PHASE
IS DIFFERENT IN NORMAL PHASE AND WE USE MOSTLY REVERSE
PHASE CHROMATOGRAPHIC TECHNIQUES FOR ANALYSING SAMPLES AND
OTHER CONDITIONS REMAIN ALMOST SAME FOR NORMAL PHASE
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / b srinu
HPLC Calibration is a parameter of instrument performance in normal phase we changed column and mobile phase.
normal phase calibration not required. if we monitoring normal phase we can perform normal phase calibration.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / sandeepkatakam
due to cost effective we are doing in RP.otherwise we can
do in both
| Is This Answer Correct ? | 4 Yes | 6 No |
Answer / zahir
In reverse phase it is easy to calibrate hplc than normal phase bec in normal phase n haxane is used it v highly evapurate so it also change in the area of the sample as time passes and n hexane is break septa of vial so we preferred reverse phase than normal phase.
| Is This Answer Correct ? | 0 Yes | 4 No |
Answer / oumspn
coz caffeine is nonpolar & we have to take rp hplc
| Is This Answer Correct ? | 0 Yes | 8 No |
what is dissolution
what is mean by nitrosamine impurities?
why we should take dst factor for below 1%moisture samples
0 Answers Ind Swift Laboratories,
How we develop a method by HPLC for unknown compound,if we got three peaks with same response then how we can find out which one is our desired compound's peak without taking help of LC MS.
what is the difference between BDS and ODS Columens and both are same or not
2 Answers Intas, IPQA, Micro Labs, Vasava Engineering Private Limited,
How to calculate LOD & LOQ in HPLC Validation Method
14 Answers Dr Reddys, GSK GlaxoSmithKline, Reddy Labs, Reliance, UIS,
What is the difference between the optics of UV and PDA Detectors ?
ash or residue in that what will be there.
HOw do i calculate the number of theoretical plate on a HPLC chromatography
Why we more noise in PDA detector compared to UV detector?
What is the purpose of octyl silane columns?
full name of OOS & OOT ? Dercribe it with details ? why this is needed in pharma industry ?
Organic Chemistry 
