How we develop a method by HPLC for unknown compound,if we got three peaks with same response then how we can find out which one is our desired compound's peak without taking help of LC MS.

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How we develop a method by HPLC for unknown compound,if we got three peaks with same response then h..

Answer / dinesh solanki

If it is a assay method then inject std in hplc system and compare the chromatogram and identified the interested peak from them.
Same RC method inject std and identified the interested peak and other is may be impurities or contamination of sample.

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How we develop a method by HPLC for unknown compound,if we got three peaks with same response then h..

Answer / shashikant

We can interprit the peaks on the base of polarity of related impurities

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How we develop a method by HPLC for unknown compound,if we got three peaks with same response then h..

Answer / dkreddy

Run In PDA Detector and Compare The Spectras.

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How we develop a method by HPLC for unknown compound,if we got three peaks with same response then h..

Answer / chennusrinivas

Based on drug Melting point we wil identity that particular
drug, from that will inject different concentration sample .

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