Answer / siri

We can use the following formula for calculation of the Theoretical plates(N).


N = 16*[TR/W]2

Here N = Theoretical plates
TR = Retention Time
W = Peak width

Answer / daljit singh randhawa

Theoretical plates(N):-

N = 5.55 x [RT/W]2

Here N = No. of Theoretical plates
RT = Retention Time
W = Peak width at half the hight of peak


Suppose RT of peak is 20 min. & Hight is 10 mm

Then W = 10/2 = 5


so N = 5.55 x [20/5]2

= 5.55 x [4]2
= 5.55 x 16 = 88.80

plz tall me that any pharmacopeia and guideline is par meted to use 1.68,4.01,7.00,9.00,12.45 buffer for ph meter calibration

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