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HPLC Calibration was done in Reverse Phase, but not in
normal phase why explain?
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Answer Posted / achyut
calibration -related with the total performance of hplc system
pump,flow , injection , accuracy & so on
in case of reverse phase, system suitability performed with well accuracy & precision because in rp volatile organic
phases are not used so the chances of getting accuacy is more
than np
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