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HPLC Calibration was done in Reverse Phase, but not in
normal phase why explain?
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Answer Posted / prabhakaran.g.s
Reverse phase and Normal phase classification is based on
the mobile phase and stationary phase (column).But we're
calibrating the HPLC for PUMP,COLUMN OVEN,AUTOINJECTOR and
DETECTOR etc.So we can perform the calibration for HPLC
with Normal phase also.But the ease availability of column
and mobile phase of Reversed phase, it is widely use for
the HPLC calibration.
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