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HPLC Calibration was done in Reverse Phase, but not in
normal phase why explain?
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Answer Posted / deepak pal
WE CALIBRATE HPLC FOR REVERSE PHASE BCOZ ONLY MOBILE PHASE
IS DIFFERENT IN NORMAL PHASE AND WE USE MOSTLY REVERSE
PHASE CHROMATOGRAPHIC TECHNIQUES FOR ANALYSING SAMPLES AND
OTHER CONDITIONS REMAIN ALMOST SAME FOR NORMAL PHASE
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