HPLC Calibration was done in Reverse Phase, but not in
normal phase why explain?
Answer Posted / oumspn
coz caffeine is nonpolar & we have to take rp hplc
| Is This Answer Correct ? | 0 Yes | 8 No |
Post New Answer View All Answers
How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?
How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?
How do we quantify crystaline and amarpous forms by using (NMR, XRD)spectroscopic techniques? Which any others instruments are useful for this quantification? explain
How you develop a method in HPLC?
in which situation require to change rs specification?
How we choose the mobile phase for method development.?
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
How to establish relative response factor for hplc. Why it is required?
what is turbidimetric titration?Give 2 examples.
in which situation ion pair agent require to use?
What is the diffrence in japan mkt requirement in analytical method validation over US?
How to choose the which salt is suitable for mobilephase
for which product require to do content uniformity? what is limit of cu?
How do we fix the sample concentaryion in hplc method development. What is the basis?
if instrument calibration fails then what require to do?