Interested to Buy Any Domain ? << Click Here >> for more details...
Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
- 205107 Views
- Arvind, Aurobindo, Cipla, Reddy Labs, Shasun Chemicals, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / pranav desai
1#It is primary reference stanard.
2#It is suggested in US,IcH Pharmacopiea.
3#It is 2 Mixima and 1 Minima
(205nm,273nm)(245nm)
4#it is eslyli soluble in solvent.
5# it is less carry over.
| Is This Answer Correct ? | 1 Yes | 2 No |
Answer / ravi kiran
it will detect at dual wave length and easily available in
market not degrade
| Is This Answer Correct ? | 0 Yes | 1 No |
Answer / satishchandra m.patel
2 maximas
and 1 minima
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
also suggested in pharmacopiea
caffeine easily available in marketIt is easily soluble in
water
| Is This Answer Correct ? | 1 Yes | 3 No |
Answer / k.g.gupta dr.reddys
Most of the Pharmaceutical products are analysing in the
range of 200-300nm. Caffiene is having 2maxima and 1
minima.It is very stable molecule.
| Is This Answer Correct ? | 2 Yes | 4 No |
Answer / b.naga vara lakshmi
1.IT SHOW MAXIMUM WAVELENGTH, AND ALSO PRIMARI REFERENCE
STANDARD,
2.IT PHYSICALLY STABLE IN NATURE AND USE LONG TIME,
3.IT IS FREELY SOLUBLE IN ANY SOLVENT.
| Is This Answer Correct ? | 0 Yes | 2 No |
Answer / suresh nv
easily avalable inmarket give good resolution
| Is This Answer Correct ? | 3 Yes | 7 No |
Answer / shivakumar
Caffeine is used for HPLC calibration in detector wavelength
accuracy test just because.
-it is completely soluble in water.
-it have a range of absorption wavelengths.
max at 205nm minima at 245nm and a maxima at 273nm.
- more over it is very stable for long time even in
solution(water).
| Is This Answer Correct ? | 3 Yes | 8 No |
Answer / vikram kodre
along with above answers very important think is caffeine
extracted from nature, so it get in highly pure form.
| Is This Answer Correct ? | 15 Yes | 25 No |
Answer / ajay
caffiene in needle shape easily dissolve in methanol &
methanol is very chip
| Is This Answer Correct ? | 11 Yes | 44 No |
Answer / vaibhav jain
Its somethng chromphore group which is caffeine having i m
nt surely tell bt whnvr i m sure i wl post answer
| Is This Answer Correct ? | 28 Yes | 104 No |
I want analyze my compound at 325 nm so which standard shall i use to calibrate my HPLC system? How i can assure my hplc detector is suitable or not?
What is viod volume and peak purity in HPLC?
if identification threshold crosses the limits then what next step?
Difference between standard operating procedure and standard test procedures in pharmaceutical industry
How to compare XRD graphs against standard and carry polymorpism study of API's by powder XRD method?
Please give idea about method development for cleaning method and how maco level establish
If combination product how require to identify which imp is of which api?
how to prepare 25 ppm acetone. explian with formula
What is descriminatory disolution medium ?
3 Answers Cipla, Graviti Pharmaceuticals,
How to calculate LOD & LOQ in HPLC Validation Method
14 Answers Dr Reddys, GSK GlaxoSmithKline, Reddy Labs, Reliance, UIS,
which detector is used in IR
how to pepare 6.8 pH phosphate buffer
Organic Chemistry 
