Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer Posted / pranav desai

1#It is primary reference stanard.
2#It is suggested in US,IcH Pharmacopiea.
3#It is 2 Mixima and 1 Minima
(205nm,273nm)(245nm)
4#it is eslyli soluble in solvent.
5# it is less carry over.

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