I want analyze my compound at 325 nm so which standard
shall i use to calibrate my HPLC system? How i can assure
my hplc detector is suitable or not?
Answers were Sorted based on User's Feedback
Answer / jd
For calibration use caffine.
And for hplc detector u calibrate the detector by make 5 lenear solution.And calculate co-relation co-officent it should not less than 0.99
Is This Answer Correct ? | 17 Yes | 3 No |
Answer / sandranagarjuna
While we performing wavelength accuracy we can use pyrine as a standard which having absorbable maxima at 318 nm and 333 nm
Is This Answer Correct ? | 2 Yes | 0 No |
pda detector mechanism?
1 Answers DKR, Teva Pharmaceuticals,
Why caffeine as a reference standard used for calibration of HPLC?
11 Answers Gangwal Chemicals, Orchid,
what is pK & pKa value
The boiling point of 1,3 butadiene is -4.4 DC & the boiling point of Pentane is 36.1 DC. Then how come Pentane elute before 1,3 BD in GC analysis?
what are risk assement in the analytical qbd?
Why irmoisture balance is used for corrugated box moisture testing
what is the identification test of volatile substance? what is volatile compound?
Which parameter require to do for analytical method equivalency?
what is the effect of injection volume calculate flow rate in perkin elmer headspace? (ccm parameter in HS)
why octanol used to determine the partition coefficient ?
what does pH stand for?
why we have to store the pH electrode in 4.01 buffer ?
1 Answers Aurobindo, BioMax, BioSynth, Cipla, Indoco Remedies, SGS,