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how to pepare 6.8 pH phosphate buffer

Answers were Sorted based on User's Feedback



how to pepare 6.8 pH phosphate buffer..

Answer / dinesh

Add 6.8 gm KH2PO4 and about 0.94 gm NaOH in 1000mL Water.
It directly shows pH 6.8.
Adjust the pH of solution by using dilute Orthophosphoric
acid or NaOH.

6.8gm KH2PO4 + 0.94gm NaOH

Is This Answer Correct ?    121 Yes 28 No

how to pepare 6.8 pH phosphate buffer..

Answer / k v ravikishore

Take 6.8g of potassium dihydrogen phosphate (KH2PO4) and dissolve it in 1000mL of water by stirring and add 0.9g of NAOH mix it well. it will shows exactly pH 6.8 no need to adjust pH with other chemicals........

Is This Answer Correct ?    41 Yes 8 No

how to pepare 6.8 pH phosphate buffer..

Answer / pavan.rudhrabatla

according to usp
0.2M NaoH perlitre added 112mland 0.2M Kh2po4 250ml per
litre added and mixed very well

Is This Answer Correct ?    26 Yes 8 No

how to pepare 6.8 pH phosphate buffer..

Answer / swathi

Take 0.01M KH2PO4 (1.36g) in 1000mL water and sonicate it
properly and check the pH and adjust the pH to 6.8 by using
Orthophosphoric acid if pH is more than 6.8,or use KOH or
NaOh if pH is less than 6.8

Is This Answer Correct ?    17 Yes 8 No

how to pepare 6.8 pH phosphate buffer..

Answer / sandeep singh tomar

take 6.8 gm pottasium dihydrigeh phosphate +1000ml distilled
water. now take .8gm NaOH IN 100ML WATER ,now adjust the pH
of prepared buffer with these 0.2 N NaOH.

Is This Answer Correct ?    13 Yes 7 No

how to pepare 6.8 pH phosphate buffer..

Answer / rajeev

Mixed Phosphate Buffer pH 6.8 As Per IP :- 28.80 gm of disodium hydrogen phosphate and 11.45 gm of Potassium dihydrogen phosphate in 1000 ml with water.

Is This Answer Correct ?    6 Yes 0 No

how to pepare 6.8 pH phosphate buffer..

Answer / thamer

For Phosphate Buffer pH 6.8, Mixed :: Dissolve 28.80 g of disodium hydrogen orthophosphate and 11.45 g of potassium dihydrogen orthophosphate in sufficient water to produce 1000 ml.
<or>

Citro-phosphate Buffer pH 6.8
Citro-phosphate Buffer pH 6.8 Phosphate buffer solution pH 6.8
Mix 77.3 ml of a 7.15% w/v solution of disodium hydrogen orthophosphate with 22.7 ml of a 2.1% w/v solution of citric acid .

Is This Answer Correct ?    2 Yes 0 No

how to pepare 6.8 pH phosphate buffer..

Answer / mr felix

Weigh accurately 6.80g(±0.001g) of potassium dihydrogen phosphate (KH2PO4) and dissolve it in 1L-volumetric flask which contains already like 300mL of pure water ; then weigh accurately 0.90g(±0.001g) of NaOH that you transfer into the same flask, shakes well to homogenize the solution. The last step is all about topping up to the volume. surely that prepared solution will have exactly pH 6.8 and there in no need to adjust pH with other chemicals. Thanks my colleagues scientists, (remember that this procedure is not the only one that is correct, it depends on some factors like field of application,...

Is This Answer Correct ?    0 Yes 0 No

how to pepare 6.8 pH phosphate buffer..

Answer / surendra bisen

Kh2po4 is 1.78g and na2hpo4 is 1.68g on preveualy dried at 120°c and mix 500 ml volumetric flask and messure the buffer 6.86 pH

Is This Answer Correct ?    0 Yes 0 No

how to pepare 6.8 pH phosphate buffer..

Answer / uv

take 0.02M K2HPO4+0.005M 1-OCTANE sulponic acid in 1000 ml
water adjuct ph 6.8 orthophoric acid

Is This Answer Correct ?    5 Yes 15 No

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