Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / shivakumar
Caffeine is used for HPLC calibration in detector wavelength
accuracy test just because.
-it is completely soluble in water.
-it have a range of absorption wavelengths.
max at 205nm minima at 245nm and a maxima at 273nm.
- more over it is very stable for long time even in
solution(water).
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