Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
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Answer / eapen
Along with the above mentions
1.It can be seperated in any RP columns.
2.It is a highly polar compound.It elutes readily in a
reverse phase column. So less time is required.
3. It is highly stable in solutions for a longer time.
4. It is recommended to use Theobromine also in Detector
wavelength accuracy check.
| Is This Answer Correct ? | 14 Yes | 2 No |
Answer / karunakar reddy > elico ltd
> 4 caffeiene having different wavelents it having 2
maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.
| Is This Answer Correct ? | 12 Yes | 2 No |
Answer / p.dharmakkannu
Caffeine basically having two maxima, lower wave length at
about 204nm and higher wave length at about 272nm. 1 minima
at about 242nm in methanol or mixture of methanol and water
combination(50%). It is nothing costly and easily soluble.
Maximum of the UV range products will be analyzed in this nm
range only.
it is also a primary reference standard officially in
pharmacopoeia for melting point.
If it is primary standard we will have certificate for the
caffeine. The same certificate will be used for both the
purpose.
| Is This Answer Correct ? | 7 Yes | 2 No |
Answer / rajesh
caffeine is a universal primary standard and highly
stability nature.
| Is This Answer Correct ? | 6 Yes | 1 No |
Answer / kondam.manoher reddy
caffeiene having different wavelenths it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.it contains non recemic CHON.It is very stable,
we can use a long time for analysis
it is not degradable.
| Is This Answer Correct ? | 6 Yes | 1 No |
Answer / kishanpal singh fare labs gurg
It is a primary standard and it will detect at dual wave
length
| Is This Answer Correct ? | 9 Yes | 5 No |
Answer / ravindra surdikar
Caffeine gives multiple wavelength responce
i.e. 205nm,245nm & 273nm
and it is stable and not degradable for a long time.
| Is This Answer Correct ? | 4 Yes | 0 No |
Answer / manikyam
It will give maxima at 273nm and 205nm and also give minima at 245nm. caffeine fastely ellute in any mobilephase like water or methanol.
| Is This Answer Correct ? | 4 Yes | 0 No |
Answer / ravinder reddy
Caffeine is very stable compound and it is not degraded
it will give minimum and maximum wavelengths
it is easily soluble in methanol and acetonitrile (1 : 1)
it will get any c18 columns
| Is This Answer Correct ? | 5 Yes | 2 No |
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Organic Chemistry 
