injection volume accruacy test i calibration of hplc

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injection volume accruacy test i calibration of hplc..

Injection volume is linear to the concetration of analyte.

the area of the peak will be increased, if the injection
volume is increased in linear way.

 Is This Answer Correct ? 10 Yes 2 No

injection volume accruacy test i calibration of hplc..

Connect union in to the HPLC.
Mobile phase: Methanol: Water (50:50)
Temperature : 25°C
Retention time :1min
Flow rate :0.5mL/min
Take one HPLC vial. Fill it with water. Weigh it (W1)
Make 6 injections with 50µl injection volume
Again weigh it (W2)
Mean volume = (W1-W2)/6*100 = µl

Limit = +-2% of the injection volume

 Is This Answer Correct ? 7 Yes 3 No

injection volume accruacy test i calibration of hplc..

In this calibration water factor (0.99602)to be used to
convert the weight in volume (micro liter)
Calculation should be as follows

Mean volume = (W1-W2)/6*0.99602 = µl

Limit = +-2% of the injection volume

 Is This Answer Correct ? 3 Yes 5 No

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