injection volume accruacy test i calibration of hplc




Answers were Sorted based on User's Feedback



injection volume accruacy test i calibration of hplc..

Answer / mr.bujji reddy kanchi

Injection volume is linear to the concetration of analyte.

the area of the peak will be increased, if the injection
volume is increased in linear way.

Is This Answer Correct ?    10 Yes 2 No

injection volume accruacy test i calibration of hplc..

Answer / pratima bhandarkar

Connect union in to the HPLC.
Mobile phase: Methanol: Water (50:50)
Temperature : 25°C
Retention time :1min
Flow rate :0.5mL/min
Take one HPLC vial. Fill it with water. Weigh it (W1)
Make 6 injections with 50µl injection volume
Again weigh it (W2)
Mean volume = (W1-W2)/6*100 = µl

Limit = +-2% of the injection volume

Is This Answer Correct ?    7 Yes 3 No

injection volume accruacy test i calibration of hplc..

Answer / ravi

In this calibration water factor (0.99602)to be used to
convert the weight in volume (micro liter)
Calculation should be as follows

Mean volume = (W1-W2)/6*0.99602 = µl

Limit = +-2% of the injection volume

Is This Answer Correct ?    3 Yes 5 No



Post New Answer



More Analytical Chemistry Interview Questions

if partition coefficient value was positive means, what it indicates ?

1 Answers  


what is the identification test of volatile substance? what is volatile compound?

1 Answers  


Tell me something about Vitamin A test method by HPLC

0 Answers   Lupin,


Tell me about analytical method validation in QC

0 Answers   Petronas,


2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.

0 Answers  






For LOD and LOQ determination which method is accurate? (slopemethod or s/n ratio method)

4 Answers   Reddy Labs,


iam using ph instrument lab india make.3ponit and 5point.in calibration time 3point slope will come 98.89and 96.87.that limit is 85 to 105.what basis slope will come.any calucalation between them.5point wiicome slope 99.89?why 3p0int came 2 values

1 Answers  


what is relationship between absorption and transmittance in u.v.?

11 Answers   Orchid,


what is the limit of Correlation for IR test compare to the standard and test

3 Answers   Nakoda Chemicals,


IF HPLC CALIBRATION FAILED THEN WHAT YOU DO

6 Answers  


How do u differentiate primary

1 Answers  


what is the general mechanism of high performance liqiud chromatography?

7 Answers   Amneal Pharmaceuticals, Ajanta Pharma,






Categories
  • Organic Chemistry Interview Questions Organic Chemistry (289)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (113)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1223)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (302)
  • Chemistry AllOther Interview Questions Chemistry AllOther (77)