injection volume accruacy test i calibration of hplc
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Answer / mr.bujji reddy kanchi
Injection volume is linear to the concetration of analyte.
the area of the peak will be increased, if the injection
volume is increased in linear way.
| Is This Answer Correct ? | 10 Yes | 2 No |
Answer / pratima bhandarkar
Connect union in to the HPLC.
Mobile phase: Methanol: Water (50:50)
Temperature : 25°C
Retention time :1min
Flow rate :0.5mL/min
Take one HPLC vial. Fill it with water. Weigh it (W1)
Make 6 injections with 50µl injection volume
Again weigh it (W2)
Mean volume = (W1-W2)/6*100 = µl
Limit = +-2% of the injection volume
| Is This Answer Correct ? | 8 Yes | 3 No |
Answer / ravi
In this calibration water factor (0.99602)to be used to
convert the weight in volume (micro liter)
Calculation should be as follows
Mean volume = (W1-W2)/6*0.99602 = µl
Limit = +-2% of the injection volume
| Is This Answer Correct ? | 5 Yes | 5 No |
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