Interested to Buy Any Domain ? << Click Here >> for more details...
In my analysis by HPLC,In sample main peak and placebo
peak are eluting with shorter resolution ( almost look like
doublet). how can i get max resolution ? Is changing
gradient method is usefull?
- 3 Answers
- 12607 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / wasim shaikh
You can try one or more of the following:
1) Changing the column type (c8 to c18, etc.)
2) Changing the Mobile phase composition (increase buffer
etc.)
3) Decrease the particle size on column (from 5 to 4, 3
etc.)
4) Increase the column length
5) Decrease the flow rate
6) If all of the above fails, then as you suggested,
gradient method can be used to separate the two peaks.
P.S. : Gradient method can also be used as an alternative
to the above methods and not necessarily the ultimate
solution.
| Is This Answer Correct ? | 32 Yes | 1 No |
Answer / naveen
U CAN DECREASE THE FLOW RATE
AND INJECTION VOLUME
| Is This Answer Correct ? | 1 Yes | 1 No |
Answer / gaurav
You can increase the injection volume.Changing gradient
method is not required.
| Is This Answer Correct ? | 2 Yes | 14 No |
What is Spliit and Splitless in Gas Chromatography nad How does it work?
what is difference between assay&purity&potency?
How decided 10 ppm standard solution in Swab, rinse method.?
1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer
Explain about empower software of analysing?
one gram of which below elements has the most atoms in its? a) H b) D C)T D) He
Why red HgI2 added during starch preparation?
why inject diluted standard solution in related substance analysis by HPLC?
What is the procedure for the calibration of ph meter?
process of Diclofenac sodium,IP.
How to calculate the signal to noise ratio.
In the HPLC Calibration done wavelength accuracy done between 200nm-280nm .but not done remaining 300-400nm not done ?
Organic Chemistry 
