Answer Posted / pratima bhandarkar

Connect union in to the HPLC.
Mobile phase: Methanol: Water (50:50)
Temperature : 25°C
Retention time :1min
Flow rate :0.5mL/min
Take one HPLC vial. Fill it with water. Weigh it (W1)
Make 6 injections with 50µl injection volume
Again weigh it (W2)
Mean volume = (W1-W2)/6*100 = µl

Limit = +-2% of the injection volume

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