bujji reddy


{ City } hyderabad
< Country > india
* Profession * executive
User No # 49707
Total Questions Posted # 0
Total Answers Posted # 16

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Questions / { bujji reddy }
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Answers / { bujji reddy }

Question { Cadila, 9442 }

How to calibrate glassware against std calibrated glassware?


Answer

Designated volume, mL 10 25 50 100 250 500 1000
Limit of error, mL 0.02 0.03 0.05 0.08 0.12 0.20 0.30
Limit of error, % 0.20 0.12 0.10 0.08 0.05 0.04 0.03


Weight measurement by taking water against standard
glassware(class-A type)



Is This Answer Correct ?    2 Yes 5 No

Question { Zydus Cadila, 144127 }

What is diffrence between residue on ignition and sulphated
ash?


Answer

BOTH ARE SAME. THERE IS NO CONFUSION.JUST TERMINOLOGY
DIFFERENCE.

USP SPEAKS RESIDUE ON IGNITION
IPO SPEAKS SULPHATED ASH.

ANALYTICAL METHOD IS SAME

Is This Answer Correct ?    27 Yes 44 No





Question { 18776 }

Why we use Uracil for HPLC calibration in Detector wave
ength accuracy test?


Answer

Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.

Is This Answer Correct ?    5 Yes 8 No

Question { Cipla, 42941 }

What is the difference between Chromatograhic Purity And
Related substances


Answer

Related substances means Quantification of Specified
Impurities and unspecified impurities against Reference
standards Exclusively.

Chromatographic Purity means Unspecified impurities by area
noramlisation method.

Is This Answer Correct ?    56 Yes 12 No

Question { Aurobindo, 12365 }

what is a drug?


Answer

A DRUG SUBSTANCE CURE OR PREVENTS DISEASE OR PAIN,

IT HAS THERAPUETIC ACTION AGAINST THE LIVING BODY

Is This Answer Correct ?    0 Yes 4 No

Question { Hetero, 16808 }

when we use pda detector in hplc instead of uv detector


Answer

1.PDA Stands for PHOTO DIODE ARRAY DETECTOR.
It is Used to take Multi wavelength chromatograms.
2.The purity of the peak is good or not will be known by
PDA.
3.For Example your sample has three componets. each
componenet has different wavelength maxima. in that case it
is not possible by UV in singl injection. we can do it by
PDA Detector only.

4.In case of UV, It is dual wavelength detector only.

Is This Answer Correct ?    43 Yes 6 No

Question { 11650 }

If we are getting split peak in Chromatogram,what we can do?


Answer

The peak split involves both mobile phase and stationery
phase. The pH of the mobile phase accuracy depends.

Mainly Stationery phase deterioration. Column has to be
regenerated according to the Column certificate
recommondation solvents.

Is This Answer Correct ?    5 Yes 0 No

Question { 31788 }

difference between IR & NMR spectroscopy ?


Answer

Interaction of light with matter to know the properties of
the substance is called spectroscopy.

IR: to know the functional groups.
NMR: to know the structural elucidation. mainly used in
diagnosis and drugs.
A key feature of NMR is that the resonance frequency of a
particular substance is directly proportional to the
strength of the applied magnetic field

Is This Answer Correct ?    42 Yes 6 No

Question { 5196 }

signifcance of detector lenearity in calibration f hplc


Answer

The detector is giving Linear results means the areas of an
injection of specific concentration are relative.

that menas

if the concentration of the sample X is 0.1 %, imagine the
area is 1000.

if the concentration of X is doubled 0.2%, the area will
also be doubled that is 2000.

This relation ship is called linearity.

If the detector gives linear responses with respect to
concentrations. then the instrument works properly . then
it is calibrated.

Is This Answer Correct ?    16 Yes 0 No

Question { HLL, 11491 }

injection volume accruacy test i calibration of hplc


Answer

Injection volume is linear to the concetration of analyte.

the area of the peak will be increased, if the injection
volume is increased in linear way.

Is This Answer Correct ?    9 Yes 2 No

Question { 27538 }

what is difference between GC & GC-HS


Answer

GC-HS is useful for Residual solvents. since the sample is
heated about 40 minutes. then the volatiles present in the
sample will come to the empty space(Head space)of the vial.
then the needle will suck the vapour and inject into GC. It
means we are extracting the volatiles from the material
before injection.

In case of GC- Direct injection, sample also will go into
GC.

Is This Answer Correct ?    72 Yes 8 No

Question { 6271 }

which brands GC coloumn available in market


Answer

Agilent DB Series

DB-1,DB624,DB-5 Etc....

Is This Answer Correct ?    4 Yes 4 No

Question { 9788 }

why linerity not use in GC but use in HPLC ?


Answer

Linearity can be used in GC also.

Lineariy must be evaluated in case of analytical method
validation and calibration.

Is This Answer Correct ?    18 Yes 3 No

Question { NCL, 19815 }

How to decide restandardisation due or retest or expiry of
Volumetric solutions prepared in wet chemistry lab?


Answer

It is pre stability study of volumetric solutions. using a
standardisation.

For Eg: If you take 0.1 M Sulphuric acid, It is
standardised with sodium carbonate, we test it for 15 days
daily. the obtained molarity shuld be varied not more than
10 %. that is 0.09 to 0.11. then the expiry can be given
for 15 days.

Restandardisation period and retest period is same incase
of volumetric solutions.

Restandardisation date is decided based on the changes in
normality on testing everyday during pre stability studies.

The variation is little bit for 5 days. then we can set
restandardisation period for every 5 days.


Is This Answer Correct ?    31 Yes 7 No

Question { Dr Reddys, 44948 }

Difference between c18


Answer

the difference between these two columns is packing only
C18- octa decyl chain linked to silica.
c8- only octyl chain linked to silica.


C18 is widely used, the polymeric C18 format incorporates a
tri-functional silylation procedure whereby the octadecyl
group is bonded to 2 or 3 silica atoms on the silica gel
backbone. This increased silylation results in far greater
column stability particularly in acidic mobile phase
conditions. Stereo recognition capability is also greater
than that of the monofunctional silylation type of C18.

Is This Answer Correct ?    83 Yes 7 No

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