Answer / samresh pal

pH Play the role for ionisation and unionisation of drug molucule in mobile phase..if our drug moity is acidic nature and our mobile phase pH is acidic then our drug moity behave to unionised and unionised molecule behave like hydrophobic or nonpolar so drug moiety more attract to stationary phase( coloum) so it not eluted with mobile phase readily and mor time expend in coloum.

Answer / hitesh tank

Specific columns depends on mobile phase pH. Column life's depend on pH it should be 2 to 8 .other wise high pH mobile phase create silanol and silica get dissolved, law pH flushed out stationary phase from the columns.

Answer / hitesh tank

HPLC Analysis depend on pH ,Peak symmetry, retention time, selectivity and impurities detection, RRT,etc.

what is PH of 0.1N Hcl, 0.01N Hcl & Mehanol.

3 Answers  

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How to study genotoxicity of impurities in API?

2 Answers   NCL,

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1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer

0 Answers   Granules,

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What the difference in loss on drying and residue on ignition

2 Answers  

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what is peak purity ??

10 Answers   Cipla, Zyg Pharma,

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3 Answers  

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0 Answers   ABC,

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why salicylic acid not use for dissolution.

2 Answers   Cipla, Indo Baijin Chemicals,

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0 Answers  

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what is the working priniciple of ph, what are parts in ph meter

1 Answers  

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can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?

0 Answers   Cipla, XYZ,

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what is concentration of oxygen in U.H.P. grade N2 cylinder

1 Answers  

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