Interested to Buy Any Domain ? << Click Here >> for more details...
today i am doing calibration of HPLC. when i inject the
blank it was going on -ve.(bleow then zero) why?
- 3 Answers
- 10644 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / b.gangatharan
This is due to improper sonication of mobile phase and improper stabilisation of column.
Regards,
B.Gangatharan
| Is This Answer Correct ? | 7 Yes | 1 No |
Answer / sudheer kumar m
may be mobile phase was not stable in that particular
method. so condition the column with mobile phase and re inject.
| Is This Answer Correct ? | 2 Yes | 2 No |
Negative peak simply indicate that your mobile phase
absorbs UV light.So use only HPLC grade solvents.Even with
HPLC grade solvents you get the negative peaks you system
might not be working properly.
Regards
M.KIRAN REDDY
| Is This Answer Correct ? | 2 Yes | 7 No |
How to do regeneration of Metacarb Pb plus column?
What are the class-1 residual solvents listed in ICH guidelines?
why do we have to used saturated ammonia in TLC
One Raw material failed assay test is there any chance for failing purity of that material ?
why results are variation in same samples analysis in gc and hplc ?
Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...
How to calculate LOD & LOQ in HPLC Validation Method
14 Answers Dr Reddys, GSK GlaxoSmithKline, Reddy Labs, Reliance, UIS,
what is difference between assayand potency
which effect is mainly responsible for chromatographic separation when silica is the statinary phase at TLC? thanks in advance
why does the result variation in hplc and gc with same sample ?
what is mean by dissolution biowaiver study?
WHY pKa value is more important than pH value of the mobile phase in HPLC?
Organic Chemistry 
