Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
- 187828 Views
- Arvind, Aurobindo, Cipla, Reddy Labs, Shasun Chemicals, I also Faced
- E-Mail Answers
Answer Posted / p.dharmakkannu
Caffeine basically having two maxima, lower wave length at
about 204nm and higher wave length at about 272nm. 1 minima
at about 242nm in methanol or mixture of methanol and water
combination(50%). It is nothing costly and easily soluble.
Maximum of the UV range products will be analyzed in this nm
range only.
it is also a primary reference standard officially in
pharmacopoeia for melting point.
If it is primary standard we will have certificate for the
caffeine. The same certificate will be used for both the
purpose.
| Is This Answer Correct ? | 7 Yes | 2 No |
Post New Answer View All Answers
In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )
is it nessesary to do solution stability for 7 days?
for inorganic molecules require to do RS, Assay and disso?
which are the diffrent batches in the pharmaceuticals?
How to set analytical specification for combination products?
what is the extinction coefficient for Indomethacin or at 319nm wave length.
what is the origin to prepare standard operating procedure
what is mean by covalidation
what is partion and column chromatography
why xterra column require to use at higher ph?
for heavy metal test lead used in which form pure form or any other form for preparation of lead standard sol
What is shaking level in GC?
what is the purge flow & how to calculate
If combination product how require to identify which imp is of which api?
How require to perform linearity as per ANVISA? What are the acceptance criteria?
