In HPLC when we use Isocratic method, Gradient method in
hplc?
- 8 Answers
- 19833 Views
- Pharmaceutical, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / keerthi achanta
for method development, better to use gradient method
because we are try to develop the method by changing the
different composition of mobile phase.
once if we know the method then we can use isocretic method
| Is This Answer Correct ? | 21 Yes | 10 No |
I think
Isocrtic same composition mobile phase but some impurites not eluted
Gradient diffrent mobilephases(acn:buffer) impurites eluted good
| Is This Answer Correct ? | 4 Yes | 3 No |
Sort n simple, large no.of impurity separated in gradient but it is take laser analysis time,while in isocratic also large no.of impurity got separated but it takes too much time kp to 2 to 5 our depends compound / impurities nature.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / subbareddy
Elution strengths of different components towards mobilephase is different.Hence,elution purpose different type of mobilephases used as gradient system
| Is This Answer Correct ? | 1 Yes | 0 No |
Pick Merging problem of chromatography thats why use gradient system.
Easily elute the pick and no interference in Chromatography then use isocratic.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / ramesh artemis biotech hyd
A separation in which the mobile phase composition remains constant throughout the procedure is termed isocratic (meaning constant composition). the mobile phase composition does't have to remaian constant. A separation in which the mobile phase composition is changed during the separation process is described as a gradient elution. Then isocratic method used fro constant composition position of mobile phase and gradent method used for mobile phase is low to high concentration.
| Is This Answer Correct ? | 3 Yes | 4 No |
Answer / chandu
For large number of impurities we prefer gradient method
for better seperation.
| Is This Answer Correct ? | 3 Yes | 6 No |
what is dwell volume in HPLC?
2 Answers Aurobindo, Cadila, Strides, SUN, Sun Pharma,
priciple of gc & hplc? (properly describe)
2 Answers Cadila, Cipla, Reddy Labs,
How we will diapose UV (detector) lamp?
what is reverse phase chromarography&normal phase chromatography
why should we do limit of stray light in UV calibration and why should use potassium chloride
why water, methanol, ethanol ets solvents are used in UV SPECTRIPHOTOMETER for blank reading ?
why we use 0.1% acetone in gradient calibration of HPLC?
3 Answers Cipla, Dr Reddys, FDC,
In Number of Theoretical Plates (N) = 16 (tr/w)2. where tr: retention time, and W: peak width. My Question is here in formula what is 16.Can u explain briefly? ---Veerabhadrarao.M
3 Answers Micro Labs, Richter Themis, Sun Pharma,
How to now that my acid is contact with base or base is contact with acid in drugs, is there any techniq to identify the formation of salt(drug)
What is Endcapped Hplc column
What is the b/W dead volume and dwell volume ?
1)What's the meaning of Absorption,give a example. 2)What's the meaning of Adsorption,give a example. 2)what is the difference between Absorption and Adsorption.
0 Answers VHB Medi Life Sciences,
Organic Chemistry 
