In HPLC when we use Isocratic method, Gradient method in hplc?

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In HPLC when we use Isocratic method, Gradient method in
hplc?

Question Posted / sureshqc44

8 Answers
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In HPLC when we use Isocratic method, Gradient method in hplc?..

Answer / keerthi achanta

for method development, better to use gradient method
because we are try to develop the method by changing the
different composition of mobile phase.
once if we know the method then we can use isocretic method

Is This Answer Correct ?

21 Yes

10 No

In HPLC when we use Isocratic method, Gradient method in hplc?..

Answer / ravendranadhareddy

I think
Isocrtic same composition mobile phase but some impurites not eluted
Gradient diffrent mobilephases(acn:buffer) impurites eluted good

Is This Answer Correct ?

4 Yes

3 No

In HPLC when we use Isocratic method, Gradient method in hplc?..

Answer / hitesh_tank25

Sort n simple, large no.of impurity separated in gradient but it is take laser analysis time,while in isocratic also large no.of impurity got separated but it takes too much time kp to 2 to 5 our depends compound / impurities nature.

Is This Answer Correct ?

1 Yes

0 No

In HPLC when we use Isocratic method, Gradient method in hplc?..

Answer / subbareddy

Elution strengths of different components towards mobilephase is different.Hence,elution purpose different type of mobilephases used as gradient system

Is This Answer Correct ?

1 Yes

0 No

In HPLC when we use Isocratic method, Gradient method in hplc?..

Answer / bhavin.patel

Pick Merging problem of chromatography thats why use gradient system.
Easily elute the pick and no interference in Chromatography then use isocratic.

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0 Yes

0 No

In HPLC when we use Isocratic method, Gradient method in hplc?..

Answer / ramesh artemis biotech hyd

A separation in which the mobile phase composition remains constant throughout the procedure is termed isocratic (meaning constant composition). the mobile phase composition does't have to remaian constant. A separation in which the mobile phase composition is changed during the separation process is described as a gradient elution. Then isocratic method used fro constant composition position of mobile phase and gradent method used for mobile phase is low to high concentration.

Is This Answer Correct ?

3 Yes

4 No

In HPLC when we use Isocratic method, Gradient method in hplc?..

Answer / dkiran

in the gradient composition method , impuries give high responses upon changing the concentration of mobile phase

Is This Answer Correct ?

1 Yes

3 No

In HPLC when we use Isocratic method, Gradient method in hplc?..

Answer / chandu

For large number of impurities we prefer gradient method
for better seperation.