Interested to Buy Any Domain ? << Click Here >> for more details...
In HPLC when we use Isocratic method, Gradient method in
hplc?
- 8 Answers
- 22385 Views
- Pharmaceutical, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / keerthi achanta
for method development, better to use gradient method
because we are try to develop the method by changing the
different composition of mobile phase.
once if we know the method then we can use isocretic method
| Is This Answer Correct ? | 21 Yes | 10 No |
I think
Isocrtic same composition mobile phase but some impurites not eluted
Gradient diffrent mobilephases(acn:buffer) impurites eluted good
| Is This Answer Correct ? | 4 Yes | 3 No |
Sort n simple, large no.of impurity separated in gradient but it is take laser analysis time,while in isocratic also large no.of impurity got separated but it takes too much time kp to 2 to 5 our depends compound / impurities nature.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / subbareddy
Elution strengths of different components towards mobilephase is different.Hence,elution purpose different type of mobilephases used as gradient system
| Is This Answer Correct ? | 1 Yes | 0 No |
Pick Merging problem of chromatography thats why use gradient system.
Easily elute the pick and no interference in Chromatography then use isocratic.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / ramesh artemis biotech hyd
A separation in which the mobile phase composition remains constant throughout the procedure is termed isocratic (meaning constant composition). the mobile phase composition does't have to remaian constant. A separation in which the mobile phase composition is changed during the separation process is described as a gradient elution. Then isocratic method used fro constant composition position of mobile phase and gradent method used for mobile phase is low to high concentration.
| Is This Answer Correct ? | 3 Yes | 4 No |
Answer / chandu
For large number of impurities we prefer gradient method
for better seperation.
| Is This Answer Correct ? | 3 Yes | 6 No |
How require to perform linearity as per ANVISA? What are the acceptance criteria?
HOw do i calculate the number of theoretical plate on a HPLC chromatography
what is mean by Q in S1 and S2
In Dissolution Test why limit is define Q+5% what is the role of +5%.
In partical size by malven,which parameter is generlly change for development of dry and wet method.
How you can calculate pKa ?
How to decide the standard concentration for related substances hplc method
What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio
How to do regeneration of Metacarb Pb plus column?
for which type of applications gas chromatography is used?
5 Answers Cipla, Flamingo Pharmaceuticals,
What is aggregate and fragments in SEC?
what is reverse phase chromarography&normal phase chromatography
Organic Chemistry 
