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suresh kumar


{ City } hyderabad
< Country > india
* Profession * lab analyst
User No # 95958
Total Questions Posted # 9
Total Answers Posted # 24

Total Answers Posted for My Questions # 26
Total Views for My Questions # 89513

Users Marked my Answers as Correct # 235
Users Marked my Answers as Wrong # 54
Questions / { suresh kumar }
Questions Answers Category Views Company eMail

why we use deturium lamp in uv spctrophotometer other than lamps

1 Analytical Chemistry 8762

how to prepare 25 ppm acetone. explian with formula

4 Analytical Chemistry 14767

WHAT IS THE PURPOSE WE USE HCL, CHOPPER, BURNER, MONOCHROMATOR in AAS? PLEASE EXPLAIN BRIEFLY

Analytical Chemistry 1721

what is the working priniciple of ph, what are parts in ph meter

1 Analytical Chemistry 8634

what is optical active and non active give examples?

GVK,

1 Analytical Chemistry 3882

why kbr is used for pellet preparation in ir spectroscopy, give the reasons? why kbr shows zero dipole moment?

6 Analytical Chemistry 14270

what is diffrence between Specific optical rotation, optical rotation?

3 Analytical Chemistry 10795

In HPLC when we use Isocratic method, Gradient method in hplc?

Pharmaceutical,

8 Analytical Chemistry 18560

What is Endcapped Hplc column

2 Analytical Chemistry 8122




Answers / { suresh kumar }

Question { Amneal Pharmaceuticals, 23882 }

what is the defination of accuracy & presicion?


Answer

accuracy means the difference between true value and value
find,


preceesion means repeatability

Is This Answer Correct ?    5 Yes 0 No

Question { IPQA, 38624 }

What is the composition of K.F


Answer

kf composition is 1mole of iodine,
1mole of so2,3 moles of pyridine,1mole of meoh,we use
pyridine free kf reagent because pyridine effects the
sexual problems in humans

Is This Answer Correct ?    16 Yes 4 No


Question { Agilent, 37817 }

How are PDA Detector work in HPLC


Answer

PDA MEANS PHOTO DIODE ARRY DEYECTOR.ITS ALSO WORK LIKE UV
DETECTOR,TO FIND OUT THE RETENTION TIME OF ANALYTE PEAK AT
MULTIPLE WAVE LENTH.EX CAFEEN ITS SHOWS MAXIMUM ABSORBANCE
IN 203-207NM,276-281, AND MINIMUM ABSORBANCE 243-247 NM

Is This Answer Correct ?    2 Yes 0 No

Question { 17830 }

which brands HPLC COLUMN available in market


Answer

column is ihe heart of hplc,we use mostly c18.c8, c4,pheno
type,cyno type columns, ihis are all the non polar
stationary columns

Is This Answer Correct ?    2 Yes 0 No

Question { 32894 }

IF HPLC CALIBRATION FAILED THEN WHAT YOU DO


Answer

calibration means whether the instrument properly working
or not, when compared to reference standard.

hplc calibration fail we can raise the quality impacting
insident that means which effect on the results of your
sample.

Is This Answer Correct ?    5 Yes 6 No

Question { 17660 }

what is the diff betwen calibration and system suitability?


Answer

calibration means whether the instrument properly working
or not, when compared to reference standard based on some
parameters. if it fails it is a quality impacting insident.

system suitability means it is use full for analyst,
reagents, columns. these are intended use.

Is This Answer Correct ?    0 Yes 0 No

Question { Aurobindo, 10831 }

what is reverse phase chromarography&normal phase
chromatography


Answer

in reverse phase chromatography we use sp is non ploar,and
mp is polar ,where as in normal phase chromatography we use
sp polar, and mp is non polar,so most of the industries
follow reverse phase chromatography,because mostly organic
compounds are in polar nature and we reduce the Retention
time

Is This Answer Correct ?    2 Yes 0 No

Question { 8740 }

what is mean by UOD?


Answer

uod means uniformity of dosage. that means we take 10
tablets in any solvent and dissolving the tablets in
solvents,and measure the assay in each tablet. in all
tablets the assay is equal is equal.

Is This Answer Correct ?    3 Yes 3 No

Question { Gennex, 24598 }

What is difference between oos


Answer

oos means out of specifications.when during the analysis of
sample ,when sample fail we raise the oos,and further
investigation.

deviation means when we deviate the approved proedure like
sop,stp

Is This Answer Correct ?    8 Yes 0 No

Question { Cipla, 20287 }

why do we adjusted ph in the mobilphase


Answer

because we reduce the retention time of main peak

Is This Answer Correct ?    4 Yes 8 No

Question { 8265 }

is there any exact definition for Theoritical plates ?
chennu srinivas


Answer

Theretical plates means the rate of absorbance is equal to
rate of disorption . accepatance cretiera nlt 2500 as per usp.


formula: TR2-TR1/ O.5*TW1-TW2.

TR2= RT OF FIRST PEAK, TR1= RT OF SECOND PEAK,

TW1= PEAK WIDTH OF FIRST PEAK, TW2= PEAK WIDTH OF SECOND PEAK

AS PER USP THIS FORMULA

Is This Answer Correct ?    1 Yes 6 No

Question { 4418 }

basic of hplc,columns used


Answer

hplc means high performance liquid chromatography, there
are two types in hplc. normal, reverse phase.
we use only reverse phase because to reduce retention time.

in hplc we use C18,C8,C4,pheno type, cyano type columns in
hplc. carbon chain increses non polarity also increases.

C18 is more non polar than C4

Is This Answer Correct ?    3 Yes 0 No

Question { Cipla, 8316 }

what are the limit of LOD and LOQ


Answer

limit of LOD: SIGNAL/ NOISE RATIO= 3.

limit of LOQ: SIGNAL/NOISE RATIO=10

Is This Answer Correct ?    12 Yes 3 No

Question { 10888 }

Why do we need GC instead of HPLC? or Why do we need HPLC
instead of GC?


Answer

in hplc compounds are seperate based on adsorption, in this
technique less volatile compounds are seperated, column are
made from silica.


in gc compounds are seperate based on partition, high
volatile compounds are seperated, capiilary columns are used

Is This Answer Correct ?    2 Yes 2 No

Question { Nectar Lifesciences, 9192 }

What is the difference of time between two consecutive injections in HPLC?


Answer

theare are two consecutive injcetors in HPLC. auto
injection, manual injection.


in auto injcection the samples are placed in tray its
having 100 above vials holes, where that saples are placed.


in manual the samples are injected buy syrangy in vials

Is This Answer Correct ?    0 Yes 11 No

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