Why do we need GC instead of HPLC? or Why do we need HPLC
instead of GC?
Answers were Sorted based on User's Feedback
Answer / a.prabhakar
GC: Is used for analysis of volatile compounds and thermal stable, (detectors are FID,TCD,ECD and pCD i.e...)volatility is important for GC.
HPLC : Non volatile and compounds having chromophores is used for HPLC (Here voltatile nature is not important both volatile and non volatile compounds are analysd on HPLC but volatile nature is very important to GC.
Is This Answer Correct ? | 8 Yes | 0 No |
in hplc compounds are seperate based on adsorption, in this
technique less volatile compounds are seperated, column are
made from silica.
in gc compounds are seperate based on partition, high
volatile compounds are seperated, capiilary columns are used
Is This Answer Correct ? | 2 Yes | 2 No |
Answer / punnitharaja
Gc analyze only for residual solvents means here using
flame ionization detector at a time so many solvents
analyzing temperature program also here.HPLC at a time not
using so many solvents because here column using polar or
non polar but GC using like midpolar also. Main reason is
dissolving process in mobile phase because polar and
nonpolar nature
Is This Answer Correct ? | 0 Yes | 2 No |
GC is used for the analysis of solvents only,which HPLC can
not analyse.
Accuracy is more in GC in comparision to HPLC.
Is This Answer Correct ? | 6 Yes | 15 No |
how can we determine RRF value in HPLC FOR CALCULATION OF IMPURITY ?
what is the difference between the ordinary loss on drying technique and vacuum oven technique?
one gram of which below elements has the most atoms in its? a) H b) D C)T D) He
what is the difference between c-8 and c-18 column
why do u get different drug release while doing calibration of dissolution test appratus 1& 2(paddle & basket)?
1 Answers HDFC, Medrich, Twilight Litaka Pharma,
How do we fix the sample concentaryion in hplc method development. what is the basis?Thanks in advance
4 Answers DRL, Glenmark, Orchid,
How will u perform degradation study in detectors other thann PDA in HPLC Methodology
Why pyrene is also use for calibration if detector
inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?
One Raw material failed assay test is there any chance for failing purity of that material ?
why we calibrate ph meter and balance daily.....but hplc and uv requires quaterly?
how to calculate elemental impurities?