In which situation we require to prepare the standard solution from sample in Related substance method?
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Answer / Jitendra Singh Hyanki
In the related substance (RS) method, a standard solution is prepared from an authentic reference standard of the related substance. This is necessary when: 1. Calibrating and validating the analytical method: Preparing the standard solution ensures that the method is accurate and precise for measuring the related substance. 2. Quantifying the amount of the related substance in a sample: Once the method has been validated, a known quantity of the standard solution can be used to calculate the concentration of the related substance in a sample.
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