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how to convert normal phase hplc to reverse phase and vice versa?
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Answer / anil suryawanshi
For normal phase system to reverse phase conversion ,,, 1st we use IPA solvent> Ethanol /Methanol> water: methanol 1:1> Water 100% ....
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hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
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