how to convert normal phase hplc to reverse phase and vice versa?
Answer / anil suryawanshi
For normal phase system to reverse phase conversion ,,, 1st we use IPA solvent> Ethanol /Methanol> water: methanol 1:1> Water 100% ....
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why 0.005 M sulphuric acid is used to calbrate spectrophotometer and why caffiene is used for calibrating hplc
2 Answers GVK, Torrent Pharma,
in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?
WHAT IS UNIT OF ABSORBANCE AREA IN HPLC OR UV DETECTER
why in uv we take k value less than 1
WHY POTASSIUM HYDROGEN PTHALATE IS USED FOR PERCHLORIC ACID? What is difference btw UV detector & RID detector? what is dissolution? What is unit of KF? what is the significant of pH in HPlc?
Defination of ZEEMAN EFFECT in NMR ?
How we can identify process related and degradation impurity in single method with short period?
why water, methanol, ethanol ets solvents are used in UV SPECTRIPHOTOMETER for blank reading ?
what is role of pH in mobile phase
3 Answers Cipla, Dhanuka Laboratories,
How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.
In the test for control of absorbance in UV calibration why do we use only potassium dichromate and what is purpose of taking a specified amount of 57.0-63.0mg?
8 Answers Aurobindo, Cipla, FDC,
why require to do water content for drug product?