What is the difference of time between two consecutive injections in HPLC?
Answers were Sorted based on User's Feedback
Answer / fionahu
There are several reasons for the time difference between two consecutive injections in HPLC. Based on Van Deemter equation, the plate height is dependent on the linear velocity of the mobile phase , multiple path effects ( eddy diffusion), longitudinal diffusion, and mass transfer between mobile phase and stationary phase. All of these factors above could result in peak broadening in LC chromatogram, which shows as elution time differences between different injection of the same samples.
Is This Answer Correct ? | 1 Yes | 1 No |
theare are two consecutive injcetors in HPLC. auto
injection, manual injection.
in auto injcection the samples are placed in tray its
having 100 above vials holes, where that saples are placed.
in manual the samples are injected buy syrangy in vials
Is This Answer Correct ? | 0 Yes | 11 No |
what does optical rotation and specific rotataion means,difference between them
solvents used in NMR
What is the difference between Optical rotation and Spcefic optical rotation
What is vial equlibriation in Gc?
Why base line not start from zero in GC Chromatography analysis.
is ther any guidline is available for limt of peak fronting.and what is the limt of peak fronting.
How to choose the which salt is suitable for mobilephase
What is AV value in test uniformity content ,describe calculation
What is UV cut off?
What is pooled sample in Dissolution
2 Answers Graviti Pharmaceuticals, Lupin,
why we use pyridine to determine moisture content of acetonel
in uv visible spectroscopy calibration why we are using potasium dichromate and tolune