What is the difference of time between two consecutive injections in HPLC?
Answers were Sorted based on User's Feedback
Answer / fionahu
There are several reasons for the time difference between two consecutive injections in HPLC. Based on Van Deemter equation, the plate height is dependent on the linear velocity of the mobile phase , multiple path effects ( eddy diffusion), longitudinal diffusion, and mass transfer between mobile phase and stationary phase. All of these factors above could result in peak broadening in LC chromatogram, which shows as elution time differences between different injection of the same samples.
|Is This Answer Correct ?||1 Yes||1 No|
theare are two consecutive injcetors in HPLC. auto
injection, manual injection.
in auto injcection the samples are placed in tray its
having 100 above vials holes, where that saples are placed.
in manual the samples are injected buy syrangy in vials
|Is This Answer Correct ?||0 Yes||11 No|
According to ICH,India is coming under which climatic zones?
tell me about aliphatic acid estimation by hplc which columns can we use
what is principal of karl ficher instrument!
What is difference between FID and TCD in gas chromatography
HOW TO CALIBRATE HPLC OVEN
why we use 0.1% acetone in gradient calibration of HPLC?
WHAT IS THE PURPOSE WE USE HCL, CHOPPER, BURNER, MONOCHROMATOR in AAS? PLEASE EXPLAIN BRIEFLY
WHAT IS USE OF ION PAIR REAGENTS?
how v can identify functional groups by wet techniques?
one gram of which below elements has the most atoms in its? a) H b) D C)T D) He
why there is a need of optical activity & specific optical activity measurement by polarimeter
why in GC calibration of linearity regression coefficient should not be less than 0.999