why water, methanol, ethanol ets solvents are used in UV
SPECTRIPHOTOMETER for blank reading ?
Answers were Sorted based on User's Feedback
Answer / chemdeep
usually the same solvent is used in uv for blank readings
that is used in the sample preparation.
Is This Answer Correct ? | 28 Yes | 6 No |
Answer / rama
If your medium is given solvents, then that solvents can be
used as blank other wise that can't be.
Is This Answer Correct ? | 17 Yes | 4 No |
Answer / nitesh pal
all that solvent not absorb uv light i.e not show absorbance in uv region
Is This Answer Correct ? | 3 Yes | 2 No |
Answer / nabil
Methanol for example absorbs at wavelengths below nm, and
water below 190 nm if we use methanol-water mix as a blank
we would have to use a wavelength grater than 205 nm to
avoid false reading.
Is This Answer Correct ? | 5 Yes | 8 No |
Answer / radha
they do not give any reaction with sample did n't give peaks
Is This Answer Correct ? | 7 Yes | 15 No |
if relative response factor is 1.07, to select how much value
How to select Sample concentration in RS analysis?example.
what is LC - MS ? What its wide use in cro/pharmaceutical Industry
What is the difference between kf and LOD
How to calculation of residual solvent methanol (3000 ppm) standard solution, and how many quantity used of test sample
Colorimtry
why we use holmiumperchlorate in calibrating uv
4 Answers Aurobindo, DRL, MSN Pharma,
When we calculate Impurity against impurity std.solution and when we calculate impurity against diluted standard with applying RRF?
Please do not join if u got offer in oncotherapies ltd (STRIDESARCOLAB)especially Mr.Mahesh Bhatt team because job is not secure his team and doesn't have confidence on him becareful this offer?
Why 1.2% KCl is used for limit of stray light? and why limit of stray light is required in UV calibration?
what king of work is done in Regulatory Affairs in Pharma industry ?
How to calculate lamp energy in hplc?