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what is the concept for adjustment of pH in HPLC buffer solution ?
a. Common ion effect
b. Counter ion effect
and explain me, how we adapt this principles & what is the role of these principles in our method
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Answer / chetan
To avoid peak overlaping we used buffer and it gives sharp single peak
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Principle of single pan analytical balance
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
When i inject amines and acid on HPLC then which peak will elut first?
Organic Chemistry 
