why do we adjusted ph in the mobilphase
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Answer / rajkumar
To ionise or deionise the analyte during passing of the
column. At that pH only the analyte may be in ionise form
or deionise form.
| Is This Answer Correct ? | 50 Yes | 3 No |
Answer / pharmainterview
To set sample in single form whether it may ionized form or
neutral form then only we can get good peak shpe.
Regards
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| Is This Answer Correct ? | 16 Yes | 1 No |
Answer / simhadri
It is better to adjust our mobile PH minium 1 or 2 units away from the analyte PKa,to get good peak shape and good retention.
| Is This Answer Correct ? | 8 Yes | 1 No |
Answer / vijay
The adjust ment of ph of the buffer is to purpose of a
corrert retntion time of the main peak
| Is This Answer Correct ? | 9 Yes | 8 No |
Answer / sheela p
Because at certain pH molecules are effective that's why we adjust the pH of mobile phase.
| Is This Answer Correct ? | 3 Yes | 2 No |
Answer / gopikrishna dr reddyys
at a particular ph we wil get impurity peaks and we ll take salts in the hplc water for buffer preparation that time the ph of that solution will not stable we have adjust the ph with suitable acid or base that time the conjugation bond will form then ph of buffer will stable
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / guest
The PH adujustments is depends upon the substance we r
going to use...and it will help for elute the peak
earlier..
| Is This Answer Correct ? | 0 Yes | 3 No |
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