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why do we adjusted ph in the mobilphase

Answers were Sorted based on User's Feedback



why do we adjusted ph in the mobilphase..

Answer / rajkumar

To ionise or deionise the analyte during passing of the
column. At that pH only the analyte may be in ionise form
or deionise form.

Is This Answer Correct ?    50 Yes 3 No

why do we adjusted ph in the mobilphase..

Answer / pharmainterview

To set sample in single form whether it may ionized form or
neutral form then only we can get good peak shpe.

Regards
pharmainterviewquestions.blogspot.in

Is This Answer Correct ?    16 Yes 1 No

why do we adjusted ph in the mobilphase..

Answer / simhadri

It is better to adjust our mobile PH minium 1 or 2 units away from the analyte PKa,to get good peak shape and good retention.

Is This Answer Correct ?    8 Yes 1 No

why do we adjusted ph in the mobilphase..

Answer / vijay

The adjust ment of ph of the buffer is to purpose of a
corrert retntion time of the main peak

Is This Answer Correct ?    9 Yes 8 No

why do we adjusted ph in the mobilphase..

Answer / sheela p

Because at certain pH molecules are effective that's why we adjust the pH of mobile phase.

Is This Answer Correct ?    3 Yes 2 No

why do we adjusted ph in the mobilphase..

Answer / gopikrishna dr reddyys

at a particular ph we wil get impurity peaks and we ll take salts in the hplc water for buffer preparation that time the ph of that solution will not stable we have adjust the ph with suitable acid or base that time the conjugation bond will form then ph of buffer will stable

Is This Answer Correct ?    1 Yes 0 No

why do we adjusted ph in the mobilphase..

Answer / raja.s

Ionisible compound present in substances.

Is This Answer Correct ?    0 Yes 0 No

why do we adjusted ph in the mobilphase..

Answer / guest

The PH adujustments is depends upon the substance we r
going to use...and it will help for elute the peak
earlier..

Is This Answer Correct ?    0 Yes 3 No

why do we adjusted ph in the mobilphase..

Answer / suresh

because we reduce the retention time of main peak

Is This Answer Correct ?    4 Yes 8 No

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