How will you separate Colorless compound Using Column
Chromatography?

Answers were Sorted based on User's Feedback



How will you separate Colorless compound Using Column Chromatography?..

Answer / loges

in colourless compond seperatiion each 100ml fraction
collected seperately and using Thinlayer chromatography
check the seperated fraction by using UV lamp

Is This Answer Correct ?    28 Yes 2 No

How will you separate Colorless compound Using Column Chromatography?..

Answer / dipti

i colomn chromatograph, if colorless compound has 2
seperate then, another compound is used which is colled as
coloring compound, has the ability to give color with the
compond has to detect.

Is This Answer Correct ?    27 Yes 9 No

How will you separate Colorless compound Using Column Chromatography?..

Answer / trevor molyneux

There are number of possibilities for identifying the
eluents from the column. Try passing the eluent through a
UV beam if any of the eluents fluoresce. You could also
pass the eluent through a set up with an IR source and
detector or a refractive index measuring instrument. Any
of these alternatives could be used quantitatively.

Is This Answer Correct ?    5 Yes 3 No

How will you separate Colorless compound Using Column Chromatography?..

Answer / k.madhu

WE R SEPERATED with hptlc method and some pigments is
present so i seperate colorlees

Is This Answer Correct ?    5 Yes 16 No

Post New Answer



More Analytical Chemistry Interview Questions

Dear sir, This is R.Kadambavadani working in a pharma industry. while doing RS analysis of ibuprofen API, unknown peak eluting some times in the 0.6 Min RT. Kindly send me what is the exact reason behind that. also am using ACN the mobile phase.

1 Answers  


NMR is both qualitative and quantitative?EXPLAIN

2 Answers  


what is turbidimetric titration?Give 2 examples.

0 Answers   Laurus,


what is the criteria for choosing capillary column in GC??is it dependent on sample load?

3 Answers  


Why using Holmium oxide in UV calibration

8 Answers   Orchid, Dr Reddys, Matrix, Nakoda Chemicals,






how the compound separate in coulunm,explain

0 Answers   Wockhardt,


How do you show that equal quanitities of two isomers provide the same response using a PDA

1 Answers  


How do you verify the primary,secondary and tertiary amines by IR-Spectroscopy.

2 Answers   GVK,


what is the analytical procedure for COD and BoD

1 Answers   Dai-Ichi,


The boiling point of 1,3 butadiene is -4.4 DC & the boiling point of Pentane is 36.1 DC. Then how come Pentane elute before 1,3 BD in GC analysis?

1 Answers  


What is the principle of HPLC, GC, LCMS, GCMS,LC QQQ, GC QQQ, LC Q TOF and GC Q TOF. What are the applications and Specificity?

0 Answers  


Why do we use 500ppb sucrose and 500ppb 1,4 benzoquinone in TOC calibration?

5 Answers  






Categories
  • Organic Chemistry Interview Questions Organic Chemistry (301)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1256)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (189)