Answer / loges

in colourless compond seperatiion each 100ml fraction
collected seperately and using Thinlayer chromatography
check the seperated fraction by using UV lamp

Answer / dipti

i colomn chromatograph, if colorless compound has 2
seperate then, another compound is used which is colled as
coloring compound, has the ability to give color with the
compond has to detect.

Answer / trevor molyneux

There are number of possibilities for identifying the
eluents from the column. Try passing the eluent through a
UV beam if any of the eluents fluoresce. You could also
pass the eluent through a set up with an IR source and
detector or a refractive index measuring instrument. Any
of these alternatives could be used quantitatively.

Answer / k.madhu

WE R SEPERATED with hptlc method and some pigments is
present so i seperate colorlees

what is poarimeter, why it is used in pharma,and explain briefly about polarimeter?

3 Answers   GVK,

---

How does HPLC Calculate Void volume?

1 Answers   Lupin,

---

How to establish pKa for a new API molecule ?

2 Answers   Neuland, Sun Pharma,

---

how can we decide the limit of cleaning validation? What is the criteria for it?

2 Answers   Glenmark,

---

why plane of polarized light rotated by opticaly active compunds?

1 Answers   Glenmark,

---

what type of compounds do we see lod @ wat type compounds do we see m.c

3 Answers   Orchid,

---

when we use pda detector in hplc instead of uv detector

6 Answers   Hetero,

---

What is the major difference b/w Drift and Noise

4 Answers   Aurobindo, Dr Reddys, Reddy Labs,

---

What is the diffrence in japan mkt requirement in analytical method validation over US?

0 Answers  

---

Chromatographic Purity is a Qualitative analysis

2 Answers   Shasun Chemicals,

---

From peak purity spectrum, where or at which point peak purity angle & purity threshold is checked?

1 Answers   Cipla, MSN Pharma,

---

WHAT IS UNIT OF ABSORBANCE AREA IN HPLC OR UV DETECTER

8 Answers  

---