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Difference between c18
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Answer / mr. bujji reddy kanchi
the difference between these two columns is packing only
C18- octa decyl chain linked to silica.
c8- only octyl chain linked to silica.
C18 is widely used, the polymeric C18 format incorporates a
tri-functional silylation procedure whereby the octadecyl
group is bonded to 2 or 3 silica atoms on the silica gel
backbone. This increased silylation results in far greater
column stability particularly in acidic mobile phase
conditions. Stereo recognition capability is also greater
than that of the monofunctional silylation type of C18.
| Is This Answer Correct ? | 84 Yes | 7 No |
Answer / kaleem
C18- octa decyl chain linked to silica.
c8- only octyl chain linked to silica.
-more no of carbon chain linkeges gives better
selectivy,resolution and late elution.
| Is This Answer Correct ? | 41 Yes | 7 No |
Answer / r.maxcil
amount of the carbon loaded in c18 is more than in the
column c8 and moreover c18 is good at acidic Ph
| Is This Answer Correct ? | 27 Yes | 7 No |
Answer / p.chandra sekhar reddy
IN C18 COLUMN 18 CARBONS ATTACHED TO SILICA GROUP ,AND IN C8 COLUMN 8 CARBONS ATTACHED TO SILICA GROUP,AND ALSO C18 COLUMN HAS MORE HYDROPHOBIC THAN C8 COLUMN DUE TO LONGER CHAIN OF CARBONS. C 18 WIDELY USED BCOZ IT GIVES BETTER SEPARATION.
| Is This Answer Correct ? | 21 Yes | 4 No |
Answer / shailesh wagh
IN C18 COLUMN 18 CARBONS ATTACHED TO SILICA GROUP ,AND IN
C8 COLUMN 8 CARBONS ATTACHED TO SILICA GROUP,AND ALSO C18
COLUMN HAS MORE HYDROPHOBIC THAN C8 COLUMN DUE TO LONGER
CHAIN OF CARBONS. C 18 WIDELY USED BCOZ IT GIVES BETTER
SEPARATION.
| Is This Answer Correct ? | 10 Yes | 3 No |
Answer / jeevithan
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18 & C8 reverse-phase column (particle size 3 & 5 µm) can be used to separate low molecular weight protein and peptides. Anyone can suggest me the "specification of particle size" of HPLC column for high MW protein?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? - ResearchGate. Available from: https://www.researchgate.net/post/Which_type_of_column_should_i_use_to_check_the_purity_of_high_molecular_weight_protein_using_HPLC_reverse_phase_column_chromatography [accessed Jun 27, 2015].
| Is This Answer Correct ? | 3 Yes | 0 No |
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