why we conclude the area% for the chromatographic purity?






why we conclude the area% for the chromatographic purity?..

Answer / prakash

1.the absorbance of the analyte peak should not be saturate either below or above 1000 milli absorance Unit.
2. if go for diluted standard level the analyte peak absorbance response should same compare to your high level absorbance response.
3. if you calculate the result either %area normalization or diluted standard level the result should same.
in this case, only we can proceed %area normalization method.

Is This Answer Correct ?    2 Yes 0 No

Post New Answer




More Analytical Chemistry Interview Questions

how do start hplc method development? how much sample required for developing the method development?

3 Answers   BMS, Indoco Remedies, Sun Pharma, Cipla,


Why Holium cholorate used in UV Spectro photometer?

7 Answers   Reddy Labs,


difference between calibration and validation

2 Answers   Mylan,


why water, methanol, ethanol ets solvents are used in UV SPECTRIPHOTOMETER for blank reading ?

7 Answers  


what is custom fields in waters system?

1 Answers   Aurobindo, Akorn India,






what is the diffrence between absorbance and absorption in the beer,s lamber formula ?

2 Answers  


IN pH CALIBRATION,CALIBRATION IS DONE FOR pH METER OR ELECTRODE?HOW WILL YOU JUDGE THAT THE METER TO BE CALIBRATED WITH 4,7,9.2 BUFFERS?EXPLAIN TEMPERATURE EFFECTS ON pH.

1 Answers   Aurobindo,


How precise should be the linearity in HPLC method validation?

3 Answers   Glenmark,


Why we are using only KBr in IR spectrometer why not KCl

10 Answers   Dr Reddys,


Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?

0 Answers   Ranbaxy, Apex, BNM,


Why to use propyl paraben in HPLC gradient calibration

0 Answers   Glenmark,


Why dissolution apparatus having 8 jars ?

5 Answers  






Categories
  • Organic Chemistry Interview Questions Organic Chemistry (288)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (113)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1223)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (300)
  • Chemistry AllOther Interview Questions Chemistry AllOther (77)