What is the difference between Normal phase method &
Reverse method in HPLC ? Define application of both method.
- 4 Answers
- 19569 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / k l srinivas
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.
| Is This Answer Correct ? | 57 Yes | 1 No |
Answer / maulik
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.
normal phase is based on the adsorption phenomenon and
reverse phase is based on the partition phenomenon
normal phase used for chiral compound and reverse phase used
for other
| Is This Answer Correct ? | 20 Yes | 2 No |
Answer / srikanth
above answer is perfect and also,,,....
Normal Phase: separates analytes on the basis of their
affinity for a polar stationary surface such as silica.
Reversed Phase: separates on the basis hydrophobic
interacertions, which originates from the high symmetry in
the dipolar water structure
| Is This Answer Correct ? | 7 Yes | 0 No |
Answer / ram krishna
Normal phase _Column is polar and more is non polar sudh as IPA hexane
Reversed phase_Column is non polar and more is polar such as water and acetonitrile mp
| Is This Answer Correct ? | 2 Yes | 0 No |
What should be the level/ Percentage of Arsenic in Arsenic free zinc.
0 Answers FDC, Laurus, Pharmaceutical,
why we are analyzing purity on HPLC why not in gc
3 Answers Aurobindo, Reddy Labs,
how PH an important role in HPLC separation
why using Potentometry What Purpose? differance between manual and potentiometry? why using Some of materials in usig potentiometry? What is use of mericuric acetate in Potentiometry
why we use only six bowls for dissolution of a drug
1 Answers Cipla, Natco, TelePerformance,
If we have a kc is1*10^8 and the .kp 2*10^5 of reaction A------->B C------->D what is the volume of C?
What are the six USP melting point reference standards?
What is difference between Primary and secodary reference standard?
what are the primary standards of redox titrations?Name any two.
Why noise test is performed at 254 nm in calibration of HPLC system?
When i inject amines and acid on HPLC then which peak will elut first?
formula for %RSD.
Organic Chemistry 
