What is the difference between Normal phase method &
Reverse method in HPLC ? Define application of both method.
Answer Posted / maulik
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.
normal phase is based on the adsorption phenomenon and
reverse phase is based on the partition phenomenon
normal phase used for chiral compound and reverse phase used
for other
| Is This Answer Correct ? | 20 Yes | 2 No |
Post New Answer View All Answers
what is the disso medium for tropsiun chloride
Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc
1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
What is the difference between spectro meter and spectro photo meter?
in which situation require to use paddle and basket?
Why we used in n-butyl acetate water content terminology while in ethyl acetate we used moisture content terminology?
In HPLC (Waters) software Empower1,2 &3 what is the updation for each version
What if impurity area in control sample coming more as compared to LOQ level of impurity ?
What is related substance by HPLC impurity limits as per USP?
What is the formula to determine the concentration of M of a solution given the % transmittance? Use %T = 43.7 as an example.
What is intact assay method development.....?
in gas chromatography what is the difference between gas flow rate and average linear velocity ?
Why six unit used for precision?
Why use only methanol,used determinationof moisture content not use other solvents