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What is the difference between Normal phase method &
Reverse method in HPLC ? Define application of both method.
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Answer Posted / srikanth
above answer is perfect and also,,,....
Normal Phase: separates analytes on the basis of their
affinity for a polar stationary surface such as silica.
Reversed Phase: separates on the basis hydrophobic
interacertions, which originates from the high symmetry in
the dipolar water structure
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