What is the difference between Normal phase method &
Reverse method in HPLC ? Define application of both method.

Answer Posted / ram krishna

Normal phase _Column is polar and more is non polar sudh as IPA hexane
Reversed phase_Column is non polar and more is polar such as water and acetonitrile mp

Is This Answer Correct ?    2 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

if rsd failed then what require to do?

319


for which product require to do content uniformity? what is limit of cu?

307


HI,I CLEARED BOB CLERK EXAM. MY INTERVIEW WILL BE ON 9TH OCTOBER,2010.PLEASE SEND ME INTERVIEW QUESTIONS AND ANSWERS. THANK YOU.

1732


Which is the highly polar and highly non polar column in HPLC?

1781


effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?

324






How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?

2860


How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.

2014


how much mass should be there in volumetric flask while in preparation of sample for assay? 

2053


We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy

1313


How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?

2715


sop of a uv visible spectrophotometer double beam elico model

5346


why we should take dst factor for below 1%moisture samples

2193


Why are use silicon oil mr?

738


WHAT IS THE USE OF STARTER ?????????

1904


What is intact assay method development.....?

2000