Answer / ankur dwivedi

There is no any thumb rule that the runtime should be 3
times than main peak, but also depends on the late eluting
impurities in compound; which should be confirmed at the
time of method development. Considering all these, the run
time should be selected.

Ankur Dwivedi
ZCL Chemicals, Ankleshwar

Answer / sarma dvv

In the method development, we have to extend the run time 7-
8 times more with analyte retion time. we have to check any
other peaks are eluting. In that run nopeaks are eluted
means accordingly we can fix the run time.
Generally for isocretic 3times more with resect to analyte
peak.

For Gradient we have to extend the organic concentration
maximum possible.

Answer / sundar

the runtime will be three times of main analye retention
time.

Answer / rahul

There are two three concerns to keep in mind when setting a
run time during method development.
1)Refer the degradation study data of the product.due to
this you come to know about the product beheviour with
method during stress conditions.
2)Confirm the impurity and main compund mass balance test
because of this you will assure about no further elution.
3)Run the HPLC for 5X to the column volume due to this you
can assure about and late elutions.

If with these experiments there is no elution after certain
time you can set the run time after 5 to 10mins after last
peak elution.

Answer / ajay vishwakarma

depends on the last possible impurities in compound; which
should be confirmed at the time of method development.and
degradation Study,further Run time 5.0times more W.r.t to
Analyte Peak

Answer / uvreddy

depends upon main analyte retention time .

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