Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).
Answer 89 - (a) Number of death cells = Total cells x % of death cells / 100 = 57 x 5.3 / 100 = 3.021 rounded to 3 cells. Number of viable cells = Total cells - number of death cells = 57 - 3 = 54 cells. (b) Average number of viable cells / square = 54 / 5 = 10.8 cells / square. (c) Dilution = final volume / initial volume = (100 + 50) microlitres / 100 microlitres = 1.5. (d) Concentration = (Average number of viable cells / square) x dilution x (square / volume) = 10.8 cells x 1.5 x (1 / 0.0001 mL) = 162000 cells / mL. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
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Question 58 – In the design of a solar power system steps of calculations below are followed : (a) The power output of the inverter of the of the solar panel is 100 watts. What is the power input, Pin to the inverter when the efficiency of the inverter is 50 %? (b) If the rated power of the inverter is 300 watts, how many inverter is needed for the solar panel? (c) Charge controller of V = 12 volts is used to supply power to the inverter. What is the input current I to the inverter? (d) If the charge controller capacity is 10 A, how many charge controllers are needed? (e) If a biochemical mixer consumes 100 watts, running for 2 hours per day, what is the energy consumption in kilowatt hour per day? (f) What is the input energy needed when the efficiency of the inverter is 50 %? (g) If your area receives 2.88 hours of full sunlight per day, how much energy, in kilowatt hour can be produced per day when one solar panel can produce 20 watts of power? (h) If you know that you have to produce total energy as the answer for (f), how many solar panels are needed? (i) Each V = 12 V battery has 5 ampere hours. If the total energy needed is in answer (f), then how many batteries are needed to run the biochemical mixer if without sunlight for 3 days?
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In a Laplace Transform Table, the Laplace transfer function of f(t) is F(s). When d(t) = f(t) then 1 = F(s). When x(t) = f(t) then X(s) = F(s). If d(t) is the impulse of a spring when d(t) = kx(t), then derive the equation for the impulse of a spring as X(s) in term of k. Next question : A controller has a transfer function a and the other controller has a transfer function b. The overall transfer function of both controllers is ab. What is the transfer function overall when both controllers have similar transfer function 1 / (Cs k)?
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Question 70 - According to Adolf Eugen Fick (1829 - 1901) : rate of diffusion v increases with less wall thickness t, increased area A and decreased molecular weight of a fluid M. The diffusion constant D decreased with increasing M. (a) By assuming v, t, dP, A, M and D changes proportionally of each other, find the equation of v as a function of t, dP, A and D. (b) The ratio of self diffusion constant D, at T = 273 K and P = 0.1 MPa, for gases B and C are 1.604 : 0.155. If only 2 gases exist in such a system : hydrogen and nitrogen, find the type of gas for B and C with reference to their molecular weights M. (c) By using the equation of kinetic energy 0.5 MV = constant where V = square of v, find the ratio of V for B and V for C, or V(B) / V(C), as a function of M(B) and M(C), where M(B) is molecular weight of B and M(C) the molecular weight of C : Graham's Law of Diffusion.
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