I want analyze my compound at 325 nm so which standard
shall i use to calibrate my HPLC system? How i can assure
my hplc detector is suitable or not?

Answer Posted / sandranagarjuna

While we performing wavelength accuracy we can use pyrine as a standard which having absorbable maxima at 318 nm and 333 nm

Is This Answer Correct ?    2 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

why we use Dichlorobenzene.nitrobezine.t-butyldi sulhate.for calibration of gc Head space

1779


if you have given a blank water how you analyse by general analytical techniques?

1506


How to set analytical specification for combination products?

324


what is the origin to prepare standard operating procedure

1789


What are modes of quantification in related substances

1718






Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?

2275


What is diffrence between extractable volume and deliverable volume? Answer pls

3929


What is stray light in uv what are units for stray light

1022


How can we calculate "confidence interval" in analytical method validation? Pl. explain with example.

3595


What is the difference between Discriminating media and DPDM(Dissolution Profile with Different Media)

6902


if peak get problamatic then what require to do?

317


why we should take dst factor for below 1%moisture samples

2197


Which is the highly polar and highly non polar column in HPLC?

1785


4. Describe the operation of the Craig apparatus. Chapter.3 Equilibrium Processes in Separations

2154


cefoperozone and sulbactam inj. hplc test method

2129