Answers were Sorted based on User's Feedback
Answer / kiran kumar
to maintain stable pH of the mobile phase we use buffers.
|Is This Answer Correct ?||11 Yes||2 No|
Answer / narasimhareddy
water is sensitive for change in pH that's why we are preferred buffers.buffers are resist to change in pH at certain level of addition of acids or bases
|Is This Answer Correct ?||9 Yes||2 No|
Answer / srinivas torati
in water some compounds didn't ionization thats why we
select buffers certain ph range in acid ph some compounds
ionization is good basic medium ionization less thats why
uv different in different ph's
|Is This Answer Correct ?||3 Yes||1 No|
Answer / suneel kumar
There are so many reason important are as follows..
1. To Maintain the PH of mobile Phase.
2. Most of the Pharmaceutical Substances are get easily
separation in buffer compare to water.
3. Peak shape will be good with addition of Buffer.
|Is This Answer Correct ?||1 Yes||1 No|
How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.
IF WE INCREASE FLOW RATE RT DECRESE.WHY
My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
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