why using buffers in hplc inteed of water




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why using buffers in hplc inteed of water..

Answer / kiran kumar

to maintain stable pH of the mobile phase we use buffers.

Is This Answer Correct ?    11 Yes 2 No

why using buffers in hplc inteed of water..

Answer / narasimhareddy

water is sensitive for change in pH that's why we are preferred buffers.buffers are resist to change in pH at certain level of addition of acids or bases

Is This Answer Correct ?    9 Yes 2 No

why using buffers in hplc inteed of water..

Answer / srinivas torati

in water some compounds didn't ionization thats why we
select buffers certain ph range in acid ph some compounds
ionization is good basic medium ionization less thats why
uv different in different ph's

Is This Answer Correct ?    3 Yes 1 No




why using buffers in hplc inteed of water..

Answer / suneel kumar

There are so many reason important are as follows..
1. To Maintain the PH of mobile Phase.
2. Most of the Pharmaceutical Substances are get easily
separation in buffer compare to water.
3. Peak shape will be good with addition of Buffer.

Is This Answer Correct ?    1 Yes 1 No

why using buffers in hplc inteed of water..

Answer / m.balram naidu

BUFFERS IS USED TO DEPAND UP ON THE DRUG PKa VALUES
BUFFERS PH IS ALLWAYS (+) OR (-)1
BECAUSE IS USED PHASPATE OR ACETATE BUFFERS IS USED

Is This Answer Correct ?    1 Yes 2 No

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