why using buffers in hplc inteed of water




Answers were Sorted based on User's Feedback



why using buffers in hplc inteed of water..

Answer / kiran kumar

to maintain stable pH of the mobile phase we use buffers.

Is This Answer Correct ?    11 Yes 2 No

why using buffers in hplc inteed of water..

Answer / narasimhareddy

water is sensitive for change in pH that's why we are preferred buffers.buffers are resist to change in pH at certain level of addition of acids or bases

Is This Answer Correct ?    9 Yes 2 No

why using buffers in hplc inteed of water..

Answer / srinivas torati

in water some compounds didn't ionization thats why we
select buffers certain ph range in acid ph some compounds
ionization is good basic medium ionization less thats why
uv different in different ph's

Is This Answer Correct ?    3 Yes 1 No




why using buffers in hplc inteed of water..

Answer / suneel kumar

There are so many reason important are as follows..
1. To Maintain the PH of mobile Phase.
2. Most of the Pharmaceutical Substances are get easily
separation in buffer compare to water.
3. Peak shape will be good with addition of Buffer.

Is This Answer Correct ?    1 Yes 1 No

why using buffers in hplc inteed of water..

Answer / m.balram naidu

BUFFERS IS USED TO DEPAND UP ON THE DRUG PKa VALUES
BUFFERS PH IS ALLWAYS (+) OR (-)1
BECAUSE IS USED PHASPATE OR ACETATE BUFFERS IS USED

Is This Answer Correct ?    1 Yes 2 No

Post New Answer



More Analytical Chemistry Interview Questions

cefoperozone and sulbactam inj. hplc test method

0 Answers   Graviti Pharmaceuticals,


why we use only six bowls for dissolution of a drug

1 Answers   Cipla, Natco, TelePerformance,


What are the different types of columns used in HPLC

3 Answers   Wockhardt,


viod volume and dead time

7 Answers   Oxygen Healthcare,


Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?

0 Answers   Ranbaxy, Apex, BNM,






what is a differance between assay by HPLC and assay by titration?

5 Answers  


What is the deference between Residual solvents and OVI

1 Answers   RA Chem Pharma,


What is the Calcultion of S/N Ratio.

2 Answers  


Why we use Caffeine in hplc calibration

2 Answers   Laurus, Emcure,


In kf constant term 16 what it is explain ?

2 Answers  


Why do we use 500ppb sucrose and 500ppb 1,4 benzoquinone in TOC calibration?

1 Answers  


How we fix the validity period of a Normal solution and re-standardization due date

3 Answers  






Categories
  • Organic Chemistry Interview Questions Organic Chemistry (287)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (113)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1212)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (300)
  • Chemistry AllOther Interview Questions Chemistry AllOther (77)