what is the difference between isocratic & elution technique?
- 3 Answers
- 11235 Views
- Laurus, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / p.appala raju
In isocratic same mobile phase composition is used for separation and same polarity is maintained through out the process of seaparetion.
In gradient mobile phase is lower polarity is gradually increase the polarity of the mobile phasethrough the process.
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / aditya
In isocratic technique we use single Mobile phae having same polarity throughout analysis
while in gradient technique we use more than one mobile phase having different polarities
and in gradient program the mobile phase ratio must be change with respect to time so it is flow gradient
if the mobile phase ratio was not changed with respect to time it is treated as isocratic elution
| Is This Answer Correct ? | 3 Yes | 0 No |
how the compound separate in coulunm,explain
What is column dead volume?
Why are using in Hi,zero air, Ni gass are used GC
5 Answers Aurobindo, ELBS, Vimta Labs,
When you get Moisture content by KF higher than by LOD, what does this indicate?
What is the pilot-plant in pharma sector.
what is the difference between Hydrochloric Acid & Hydrogen chloride
How require to perform linearity as per ANVISA? What are the acceptance criteria?
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
How does HPLC Calculate Void volume?
How can we calculate offset value pH measurment
What should be the level/ Percentage of Arsenic in Arsenic free zinc.
0 Answers FDC, Laurus, Pharmaceutical,
What is principle of Polarimeter?
Organic Chemistry 
