Interested to Buy Any Domain ? << Click Here >> for more details...
what is difference between assay&purity&potency?
- 5 Answers
- 33521 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / falguni
Assay : We compare the area of product with working
reference Standard of that product.It also may calculate as
basis, on anhydrous basis or on dried basis.
Purity : We compare the area of our product with area of
other impurities which comes in our product.
Potency : We compare the area of product with working
reference Standard of that product, but after removing the
sum of heavy metals, sulfated ash, LOD and moisture.
| Is This Answer Correct ? | 31 Yes | 4 No |
Answer / bhargav
Purity:
It is calculated by area normalisation method, it can be
directly find out by chromatograms obtained from HPLC.
% Purity = (Area of desired peak / Sum of area of all
peaks) x 100
Assay:
It is calculated against standard. It may be of following
types:
1) On as is basis = (Area of sample / Area of standard) x
(conc. of standard / conc. of sample) x potency or assay of
standard
2) On anhydrous basis = (Assay on as is basis / 100 –
moisture) x 100
3) On dried basis = (Assay on as is basis / 100 – LOD) x 100
Potency:
100 – (moisture or LOD + residual solvents + heavy metals
+ sulphated ash + impurities)
| Is This Answer Correct ? | 17 Yes | 2 No |
Answer / a.prabhakar
Purity :
It explains how pure our analyte is in the given mixture.
It is not related to the how much our analyte is in the given mixture.
i.e.: Percentage of a our analyte without impurities in HPLC.(Known or Unknown)
Assay:
It explains how much our analyte is in the given mixture (The content of our component in the given mixture).It is not related to analyte purity.
Potency :
It is measurement of our analyte how potent it is.
i.e: Purity of our analyte without all possible impurities like chromatographic impurities (HPLC, GC-Residual solvents, moisture, TLC), heavy metals, sulphated ash...etc
| Is This Answer Correct ? | 10 Yes | 0 No |
Answer / ala
in the answer above the first two definitions is true
for potency there is relation ship between potency and activity of the product as antibiotics and i am sure its related to micro lab
| Is This Answer Correct ? | 2 Yes | 5 No |
Describe 5 components of SFC ?
1 Answers College School Exams Tests,
In IR spectra, generally a molecule vibrates without apply emr or ir light?
How can we separate Urea & semicarbazide in single HPLC method ?
for which product require to do content uniformity? what is limit of cu?
i set electrode to 7 buffer than i used it to check 4 buffer and 10 buffer if the results are not as expected than is it better to set the buffer again to 4 buffer or is there any other method available please let me know ...
why holium oxide prepared in 10% HCLO4 SOLUTION
Why dissolution test is not performed in all of the products
how can we decide the limit of cleaning validation? What is the criteria for it?
Why we show ammonia vapours to tlc plate?to which compounds?
Which is the best mobile phase to separate organic acids using C8 column. And how should the samples be prepared for hplc?
UV and PDA detector, which have less signal to noise ratio?
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
Organic Chemistry 
