Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

what is the difference between isocratic & elution technique?

Answer Posted / aditya

In isocratic technique we use single Mobile phae having same polarity throughout analysis
while in gradient technique we use more than one mobile phase having different polarities
and in gradient program the mobile phase ratio must be change with respect to time so it is flow gradient
if the mobile phase ratio was not changed with respect to time it is treated as isocratic elution

Is This Answer Correct ?    3 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?

3020


For titration in anhydrous media with perchloric acide, if lack of titrator, Which indicator is been used for replacement. How calculate pH of test solution to choose suitable indicator?

2356


Why we check moisture,ash & AIA in product

1940


if content uniformity passing but dissolution varrying then what is next step?

936


What is stray light in uv what are units for stray light

1720


what is the structure of DOWEX-50

5561


why we should take dst factor for below 1%moisture samples

2715


What is the requirement for brazil in the analytical method vslidation comparr to ich?

849


How will u perform degradation study in detectors other thann PDA in HPLC Methodology

2597


What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio

1964


how will you do the prep for unstable componds?

2067


what is the extinction coefficient for Indomethacin or at 319nm wave length.

4125


What is the difference between chromatographic purity and related substances?

1456


How much is the standard area for glc analysis

2330


In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?

2015