Interested to Buy Any Domain ? << Click Here >> for more details...
If i performing one analysis on C8column then the same analysis done on C18 column then what will happen about Rt;Resulution;Peak shape Explain ?
- 1 Answers
- 8053 Views
- I also Faced
- E-Mail Answers
Answer / t reddy
consider ur performing combination drug.... if both the drugs hydrophilic means.
in c8 column the rt will be high compare to c18 and resolution between two peaks also will increase in c8.
i think peak shape won't affect based on this factor.
Reason - c8 is less hydrophobic than c18... because of less carbon.... c18 is more hydrophobic so the interaction between hydrophilic drug and high hydrophobic column(c18) is less, drug will elute quickly and RT will be less than c8 column and resolution also.
NOTE - this is just a possibility, u can still get the good Rt with c8 also by changing the various factors like mobile phase composition, flow rate, temperature etc
| Is This Answer Correct ? | 2 Yes | 1 No |
Why sodium hydroxide used for maintain pH of phosphate buffer
Why 1.2% KCl is used for limit of stray light? and why limit of stray light is required in UV calibration?
How can I develope method of dissolution by HPLC OR UV
In kf constant term 16 what it is explain ?
How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?
What are the six USP melting point reference standards?
why we use vanillin in melting range calibration
how would you determine the acceptance criteria for cleaning validationof product A for product B?
1 Answers Cipla, Cypher Solutions, Torrent Power,
Which type of analysis we have to done uv spectroscopy
how to select short coloum and long coloumn for new molecule
Why do we use 500ppb sucrose and 500ppb 1,4 benzoquinone in TOC calibration?
How is the Flame test performed?
Organic Chemistry 
