why we use only sucrose in the calibration of polarimeter?
Answers were Sorted based on User's Feedback
Answer / venkat
Main Reasons to use sucrose solutions :
1. Very Easily prepared
2. Has a fixed rotation value which can calibrated with 10%,
20% and 30% sugar value, and remains constant for those
concentration for a little time only, upto that we can have
a very sharp reading calibration of pure sugar.
3. Mainly polarimeter is used sucrose quality detection for
calibration with pure sucrose is the best deal.
4. Its shows almost no Muta rotation.
5. And last and final...Its most cheap to figure out and get
the calibration done.
Is This Answer Correct ? | 19 Yes | 3 No |
Answer / s.ramanjaneyulu
Because in Sucrose solution muta rotation was not present.
Its most cheap to figure out and get the calibration done
Mainly polarimeter is used sucrose quality detection for
calibration with pure sucrose.
Is This Answer Correct ? | 14 Yes | 5 No |
Answer / venkat nosch
sucrose had standard readings and did not present muta rotation
sucrose have quality detection limit
Is This Answer Correct ? | 4 Yes | 0 No |
Sucrose does not have mutarotation.so sucrose is used for polarimeter calibration.we can not use glucose&Fructose because they are having mutarotation.change in optical rotation is called mutarotation.
Thanks,
B.Gangatharan,
Caplin point laboratory,
Puducherry.
Is This Answer Correct ? | 2 Yes | 0 No |
Answer / s.giri prasad
1.Sucrose does not have mutarotation
2.It is disaccharide mixture of Glucose and Fructose.
3.It is give exact readings and very cheap.
4.It is primary standard and higly stable
Is This Answer Correct ? | 2 Yes | 0 No |
What is the function of end capped column?
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
how calibrate the hplc detector
2 Answers Cipla, Shraddha Analytical Services,
how to prepare solution in ppb
why ur using toulene in hexane in u.v calibration?
3 Answers Biological.e.limited, Cipla,
Is it compulsory to prepare KBr disc with sample in identification test by FTIR?
how you establish the LOQ?
how we can identify the impurity is coming below loq at transfering site?
what is difference between drug products and drug substance?
class of solvent regarding Residual solvent e.g. Acetone his class
defination of wavelength & wave number
analytical method validation require to with respect to release specification or shelf life specification?