What is the difference between uplc and hplc
Answers were Sorted based on User's Feedback
Answer / satheesh kumar
HPLC,UPLC both are belongs to liquid chromartography.
HPLC-Longer Run time,Pressure withstands-5000psi,low id not
UPLC-Less Run time,Pressure withstands more than
5000psi,low id possible
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UPLC is advance varsion of HPLC.
UPLC( ULTRA HIGH PERFORMANCE CHROMATOGRAPHY) is actually initiation of a new era in drug analysis with high pressure, sepration,resulution & most important speed.
uplc is patent by WATERS,whereas SHIMANDZU also on tha way by lunchimg NEXERA.
Overall UPLC is a fast version of HPLC.
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Answer / k.lavanya
HPLC is high performance liquid chromatography, whereas UPLC is ultra high performance liquid chromatography
In essence, UPLC is just a special variant of HPLC
The pump pressure, which is 40Mpa (400 atmospheres) in HPLC, can go up to 100MPa in UPLC. UPLC also allows for much smaller particle size, normally less than 2um whereas it is normally 5um in HPLC.
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Answer / khushali senghani
in UPLC productivity is increased and results are obtained mor faster with more resolution whereas in HPLC high pressure is required for solvent flow.
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