Interested to Buy Any Domain ? << Click Here >> for more details...
What is the difference between uplc and hplc
- 4 Answers
- 26475 Views
- AAU Assam Agricultural University, Hetero, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / satheesh kumar
HPLC,UPLC both are belongs to liquid chromartography.
HPLC-Longer Run time,Pressure withstands-5000psi,low id not
possible
UPLC-Less Run time,Pressure withstands more than
5000psi,low id possible
| Is This Answer Correct ? | 23 Yes | 3 No |
UPLC is advance varsion of HPLC.
UPLC( ULTRA HIGH PERFORMANCE CHROMATOGRAPHY) is actually initiation of a new era in drug analysis with high pressure, sepration,resulution & most important speed.
uplc is patent by WATERS,whereas SHIMANDZU also on tha way by lunchimg NEXERA.
Overall UPLC is a fast version of HPLC.
| Is This Answer Correct ? | 21 Yes | 4 No |
Answer / k.lavanya
HPLC is high performance liquid chromatography, whereas UPLC is ultra high performance liquid chromatography
In essence, UPLC is just a special variant of HPLC
The pump pressure, which is 40Mpa (400 atmospheres) in HPLC, can go up to 100MPa in UPLC. UPLC also allows for much smaller particle size, normally less than 2um whereas it is normally 5um in HPLC.
| Is This Answer Correct ? | 12 Yes | 1 No |
Answer / khushali senghani
in UPLC productivity is increased and results are obtained mor faster with more resolution whereas in HPLC high pressure is required for solvent flow.
| Is This Answer Correct ? | 0 Yes | 3 No |
what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?
why urasil use in hplc column perfomance?
Plz explain the formula in uv calibration =Absorbance of the sample ( x ) x 10 x 1000/Weight of potassium dichromate taken in mg
difference between record & document ?
What is the difference between the working standard and reference standard?
For Recent draft guidelines for Dissolution test apparatus calibration, why removing the salicylate tables?
why we get negative values in uv when i placed sample which has some concetration but not in standard
water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.
Is it compulsory to prepare KBr disc with sample in identification test by FTIR?
why in uv we take k value less than 1
Calculate the water content in Di Sodium Tartrate (DST) based on molecular weight?
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
Organic Chemistry 
