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How do we fix the sample concentaryion in hplc method
development. what is the basis?Thanks in advance

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How do we fix the sample concentaryion in hplc method development. what is the basis?Thanks in adv..

Answer / vipul

For Assay:- Sample concentration should be lower than higher
detector linearity concentration.
For Related substances:- Sample concentration should be
decided on the basis of LOD and LOQ determination
e.g sample concentration 1000ppm
Standard low load concentration 5ppm(For specified imp
limit-0.5%)
LOQ-should be between 0.05% To 0.09% of sample concentration.

Is This Answer Correct ?    8 Yes 2 No

How do we fix the sample concentaryion in hplc method development. what is the basis?Thanks in adv..

Answer / pramod

It is also depend upon peak response.
How ever we have to select sample concentration in rs on the basis of LOQ

"Loq should be less than Reporting threshould"

Accordign to that we have to select sample Concentration

Is This Answer Correct ?    2 Yes 0 No

How do we fix the sample concentaryion in hplc method development. what is the basis?Thanks in adv..

Answer / rajareddy

based on the peak response ,peak height and LOD and LOQ Signal. Ex:sample concentration 1mg/ml than LOQ value (0.1%mg/ml) LOD value(0.03mg/ml)

Is This Answer Correct ?    0 Yes 0 No

How do we fix the sample concentaryion in hplc method development. what is the basis?Thanks in adv..

Answer / asha latha

IF ALL IMPURTITIES AT SPEC LEVEL ARE MEETING S/N RATIO MORE
THAN 60 AT THAT PARTICULAR CONCENTRATION AS WEL AS NO
COLUMN OVERLOAD.

Is This Answer Correct ?    0 Yes 6 No

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