How do we fix the sample concentaryion in hplc method
development. what is the basis?Thanks in advance
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Answer Posted / vipul
For Assay:- Sample concentration should be lower than higher
detector linearity concentration.
For Related substances:- Sample concentration should be
decided on the basis of LOD and LOQ determination
e.g sample concentration 1000ppm
Standard low load concentration 5ppm(For specified imp
limit-0.5%)
LOQ-should be between 0.05% To 0.09% of sample concentration.
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