How do we fix the sample concentaryion in hplc method
development. what is the basis?Thanks in advance

Answer Posted / pramod

It is also depend upon peak response.
How ever we have to select sample concentration in rs on the basis of LOQ

"Loq should be less than Reporting threshould"

Accordign to that we have to select sample Concentration

Is This Answer Correct ?    2 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

iam usig ph buffers merk. manually how to prepare ?

1770


please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development

1090


What is the principle of Thermal conductivity detector and FID?

2249


Difference between hlaf and rlaf

1637


why we are using benzene, anyline in acetic anhdride assay titration method?

1565






Tell me about analytical method validation in QC

5524


1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer

1295


what is different when impergnated silica plates are used in separation of azo dyes using column chromatography?

1509


In the isomers,enantiomers give the only physical properties present such that no chemical properties present.

1765


why require to do water content for drug product?

306


Why only 1.2 million lux hours require in photostability study?

448


What is control room temperature and which guide line says?

1147


From where require to take the RLD sample?

267


what is third integration

1481


What is similarity factor and its use in api strength?

776