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What is the reason for negative peak in HPLC while using UV detector.
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Answer / ravi mishra
If your diluent is more polar than mobile phase then such negative peak appear.
| Is This Answer Correct ? | 11 Yes | 1 No |
Answer / hemesh
in UV detector cell precipitate might be formed.
it should be cleaned with hot water or diluent in which it dissolve.
| Is This Answer Correct ? | 1 Yes | 3 No |
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