What is the reason for negative peak in HPLC while using UV detector.
- 2 Answers
- 14717 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / ravi mishra
If your diluent is more polar than mobile phase then such negative peak appear.
| Is This Answer Correct ? | 11 Yes | 1 No |
Answer / hemesh
in UV detector cell precipitate might be formed.
it should be cleaned with hot water or diluent in which it dissolve.
| Is This Answer Correct ? | 1 Yes | 3 No |
What's the importance of these procedures?
How we fix the validity period of a Normal solution and re-standardization due date
Optical Rotation*0.9477*100*200] Assay Of dextrose= ________________________________ 99.8* 200 why Factor=0.9477
1 Answers Claris Lifesciences, Inven Pharmaceuticals, Levo,
Why only Copper standard is used to calibrate Atomic Absorption spectrophotometer?
in hplc chromatogram started from left to right and in uv spectrum started from left to right
what is protocol ? plz Explain me
iam usig ph buffers merk. manually how to prepare ?
why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?
What are the terms used during Melting Range Analysis ?
what is peak purity? how may types of methods to determine peak purity?
why you require QA approval & what is its importance?
How to decide restandardisation due or retest or expiry of Volumetric solutions prepared in wet chemistry lab?
Organic Chemistry 
